AP1G1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of AP1G1.
Immunogen
A synthetic peptide (conjugated with diphtheria toxoid) corresponding to amino acids 5-24 of human AP1G1.
Sequence
IRLRELIRTIRTARTQAEER
Host
Rabbit
Reactivity
Human, Rat
Specificity
This antibody has been shown to be specific to AP-1.
Form
Lyophilized
Recommend Usage
Immunofluorescence (1:200-1:2000)
Western Blot (1:1000-1:4000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry
Immunohistochemistry using DAB substrate showing intense staining in the rat airway epithelium using AP1G1 polyclonal antibody (Cat # PAB14334) at a dilution of 1 : 500. Secondary antibody was biotinylated goat and rabbit at a dilution of 1 : 3000 followed by Vector ABC. This antigen has a similar distribution to clathrin, with which it associates.Immunofluorescence
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Gene Info — AP1G1
Entrez GeneID
164Protein Accession#
O43747Gene Name
AP1G1
Gene Alias
ADTG, CLAPG1, MGC18255
Gene Description
adaptor-related protein complex 1, gamma 1 subunit
Omim ID
603533Gene Ontology
HyperlinkGene Summary
Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans-Golgi network to lysosomes. The adaptin family of proteins is composed of four classes of molecules named alpha, beta-, beta prime- and gamma- adaptins. Adaptins, together with medium and small subunits, form a heterotetrameric complex called an adaptor, whose role is to promote the formation of clathrin-coated pits and vesicles. The protein encoded by this gene is a gamma-adaptin protein and it belongs to the adaptor complexes large subunits family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
clathrin assembly protein complex 1 gamma large chain|clathrin-associated/assembly/adaptor protein, large, gamma 1|gamma adaptin|golgi adaptor HA1/AP1 adaptin gamma subunit
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Interactome
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Pathway
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Publication Reference
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Identification and characterization of novel clathrin adaptor-related proteins.
Takatsu H, Sakurai M, Shin HW, Murakami K, Nakayama K.
The Journal of Biological Chemistry 1998 Sep; 273(38):24693.
Application:IF, WB-Tr, Human, Rat, HEK 293, HL-60, Rat hepatocyte Clone 9 cells cells.
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Cloning, expression pattern, and chromosomal assignment to 16q23 of the human gamma-adaptin gene (ADTG).
Peyrard M, Parveneh S, Lagercrantz S, Ekman M, Fransson I, Sahlen S, Dumanski JP.
Genomics 1998 Jun; 50(2):275.
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Identification and characterization of novel clathrin adaptor-related proteins.
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