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Western Blot

Human embryonic kidney cells (HEK293T) were transiently transfected with an expression vector for GAL4-tagged DNMT3A.
Whole cell extracts were immunoprecipitated with 2 ug of DNMT3A polyclonal antibody (Cat # PAB14129).
The presence of GAL4-DNMT3A in the non-treated cell extracts and in the immunoprecipitates was demonstrated by western blot analysis with anti-GAL4 antibody (Lane 1 and 2 respectively).
Alternatively, GAL4-DNMT3A was immunoprecipitated and western blot analysis was performed with the DNMT3A polyclonal antibody (Cat # PAB14129) (diluted 1 : 2,000) (Lane 3).

Immunoprecipitation

Enzyme-linked Immunoabsorbent Assay

ELISA was performed using a serial dilution of DNMT3A polyclonal antibody (Cat # PAB14129), crude serum and Flow Through, in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1 : 500.

Entrez GeneID

1788

Protein Accession#

Q9Y6K1

Gene Name

DNMT3A

Gene Alias

DNMT3A2, M.HsaIIIA

Gene Description

DNA (cytosine-5-)-methyltransferase 3 alpha

Omim ID

602769

Gene Ontology

Hyperlink

Gene Summary

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq

Other Designations

DNA MTase HsaIIIA|DNA cytosine methyltransferase 3 alpha|DNA cytosine methyltransferase 3A2|DNA methyltransferase HsaIIIA|OTTHUMP00000120005|OTTHUMP00000120006