AOF2 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot was performed using AOF2 polyclonal antibody (Cat # PAB14107) diluted 1 : 1,000 in TBS-Tween containing 5% skimmed milk.
The antibody recognizes endogenous AOF2 in HeLa NE (20 ug).
Western Blot
Western blot was performed using AOF2 polyclonal antibody (Cat # PAB14107) diluted 1 : 1,000 in TBS-Tween containing 5% skimmed milk. The antibody recognizes the recombinant AOF2 (50 ng) (Lane 1) as well as endogenous AOF2 in nuclear extracts from HeLa (Lane 3) and HEK293 (Lane 4).
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant AOF2.
Immunogen
Recombinant protein corresponding to full length human AOF2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Recommend Usage
Western Blot (1:1000)
ChIP (3 ug)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide, 0.05% proclin 300)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
ChIP
Western Blot (Cell lysate)
Western blot was performed using AOF2 polyclonal antibody (Cat # PAB14107) diluted 1 : 1,000 in TBS-Tween containing 5% skimmed milk.
The antibody recognizes endogenous AOF2 in HeLa NE (20 ug).Western Blot
Western blot was performed using AOF2 polyclonal antibody (Cat # PAB14107) diluted 1 : 1,000 in TBS-Tween containing 5% skimmed milk. The antibody recognizes the recombinant AOF2 (50 ng) (Lane 1) as well as endogenous AOF2 in nuclear extracts from HeLa (Lane 3) and HEK293 (Lane 4). -
Gene Info — AOF2
Entrez GeneID
23028Protein Accession#
O60341Gene Name
AOF2
Gene Alias
BHC110, KDM1, KIAA0601, LSD1
Gene Description
amine oxidase (flavin containing) domain 2
Omim ID
609132Gene Ontology
HyperlinkGene Summary
This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
BRAF35-HDAC complex protein BHC110|FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit|lysine (K)-specific demethylase 1|lysine-specific histone demethylase 1
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Interactome
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Publication Reference
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NF-Y substitutes H2A-H2B on active cell-cycle promoters: recruitment of CoREST-KDM1 and fine-tuning of H3 methylations.
Gatta R, Mantovani R.
Nucleic Acids Research 2008 Nov; 36(20):6592.
Application：ChIP, Human, HCT-116 cells.
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NF-Y substitutes H2A-H2B on active cell-cycle promoters: recruitment of CoREST-KDM1 and fine-tuning of H3 methylations.
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