SOX4 polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
H1299 (lung carcinoma cells) were transfected with HA-tagged SOX4 (Lane 1) or with a HA-tagged truncated SOX4 (395X mutant, Lane 2).
Whole cell extracts (60~100 ug) were analysed by Western blot using SOX4 polyclonal antibody (Cat # PAB14092) diluted 1 : 4,000 in TBS-Tween containing 2% skimmed milk.
Western Blot
Whole cell extracts of 4 different lung carcinoma cell lines were analysed by Western blot with SOX4 polyclonal antibody (Cat # PAB14092) as described above.
The H522 cells are known to overexpress SOX4.
Western blot performed by Montse Sanchez-Cespedes and Sandra Castillo Diez, Catalan Institute of Oncology, Barcelona, Spain.
Enzyme-linked Immunoabsorbent Assay
ELISA was performed using a serial dilution of SOX4 polyclonal antibody (Cat # PAB14092). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 18,000.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SOX4.
Immunogen
A mixture of synthetic peptides (conjugated with KLH) corresponding to N-terminus and to C-terminus of human SOX4.
Host
Rabbit
Reactivity
Human
Form
Liquid
Recommend Usage
ELISA (1:100-1:1000)
Western Blot (1:4000)
The optimal working dilution should be determined by the end user.Storage Buffer
In serum (0.05% sodium azide)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
H1299 (lung carcinoma cells) were transfected with HA-tagged SOX4 (Lane 1) or with a HA-tagged truncated SOX4 (395X mutant, Lane 2).
Whole cell extracts (60~100 ug) were analysed by Western blot using SOX4 polyclonal antibody (Cat # PAB14092) diluted 1 : 4,000 in TBS-Tween containing 2% skimmed milk.Western Blot
Whole cell extracts of 4 different lung carcinoma cell lines were analysed by Western blot with SOX4 polyclonal antibody (Cat # PAB14092) as described above.
The H522 cells are known to overexpress SOX4.
Western blot performed by Montse Sanchez-Cespedes and Sandra Castillo Diez, Catalan Institute of Oncology, Barcelona, Spain.Enzyme-linked Immunoabsorbent Assay
ELISA was performed using a serial dilution of SOX4 polyclonal antibody (Cat # PAB14092). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 18,000. -
Gene Info — SOX4
Entrez GeneID
6659Protein Accession#
Q06945Gene Name
SOX4
Gene Alias
EVI16
Gene Description
SRY (sex determining region Y)-box 4
Omim ID
184430Gene Ontology
HyperlinkGene Summary
This intronless gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins, such as syndecan binding protein (syntenin). The protein may function in the apoptosis pathway leading to cell death as well as to tumorigenesis and may mediate downstream effects of parathyroid hormone (PTH) and PTH-related protein (PTHrP) in bone development. The solution structure has been resolved for the HMG-box of a similar mouse protein. [provided by RefSeq
Other Designations
OTTHUMP00000039358|SRY-related HMG-box gene 4|ecotropic viral integration site 16
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Interactome
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Publication Reference
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Bone morphogenetic protein and activin membrane-bound inhibitor overexpression inhibits gastric tumor cell invasion via the transforming growth factor-β/epithelial-mesenchymal transition signaling pathway.
Yuan CL, Liang R, Liu ZH, Li YQ, Luo XL, Ye JZ, Lin Y.
Experimental and Therapeutic Medicine 2018 Jun; 15(6):5422.
Application：IHC-P, WB-Tr, Human, Mouse, BGC‑823 cells, Gastric carcinoma.
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The SRY-HMG box gene, SOX4, is a target of gene amplification at chromosome 6p in lung cancer.
Medina PP, Castillo SD, Blanco S, Sanz-Garcia M, Largo C, Alvarez S, Yokota J, Gonzalez-Neira A, Benitez J, Clevers HC, Cigudosa JC, Lazo PA, Sanchez-Cespedes M.
Human Molecular Genetics 2009 Jan; 18(7):1343.
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Bone morphogenetic protein and activin membrane-bound inhibitor overexpression inhibits gastric tumor cell invasion via the transforming growth factor-β/epithelial-mesenchymal transition signaling pathway.
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