ACTL6B polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Nuclear extracts of HeLa cells (40 ug) were analysed by Western blot using ACTL6B polyclonal antibody (Cat # PAB14085) diluted 1 : 1,000 in TBS-Tween containing 5% skimmed milk.
Enzyme-linked Immunoabsorbent Assay
ELISA was performed using a serial dilution of ACTL6B polyclonal antibody (Cat # PAB14085).
The plates were coated with the peptide used for immunization of the rabbit.
By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 76,000.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ACTL6B.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human ACTL6B.
Host
Rabbit
Reactivity
Human
Form
Liquid
Recommend Usage
ELISA (1:500-1:1000)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In serum (0.05% sodium azide)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Nuclear extracts of HeLa cells (40 ug) were analysed by Western blot using ACTL6B polyclonal antibody (Cat # PAB14085) diluted 1 : 1,000 in TBS-Tween containing 5% skimmed milk.Enzyme-linked Immunoabsorbent Assay
ELISA was performed using a serial dilution of ACTL6B polyclonal antibody (Cat # PAB14085).
The plates were coated with the peptide used for immunization of the rabbit.
By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 76,000. -
Gene Info — ACTL6B
Entrez GeneID
51412Protein Accession#
O94805Gene Name
ACTL6B
Gene Alias
ACTL6, BAF53B
Gene Description
actin-like 6B
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of a family of actin-related proteins (ARPs) which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a subunit of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. This subunit may be involved in the regulation of genes by structural modulation of their chromatin, specifically in the brain. [provided by RefSeq
Other Designations
53 kDa BRG1-associated factor B|actin-like 6|actin-related protein|hArpN alpha
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Interactome
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Disease
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