ELN polyclonal antibody
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Specification
Product Description
Guinea pig polyclonal antibody raised against native ELN.
Immunogen
Native purified ELN from human skin.
Host
Guinea pig
Reactivity
Human
Specificity
This antibody reacts with human elastin 100%, cross-reactivity in RIA at 1:500 dilution: human collagen type I, II, III, IV, V <0.1 %; human fibronectin <0.1%.
Form
Lyophilized
Recommend Usage
ELISA (>1:200)
Immunohistochemistry (1:500-1000)
Immunofluorescence (1:80)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from phosphate buffered solution
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with distilled water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
Radioimmunoassay
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Gene Info — ELN
Entrez GeneID
2006Gene Name
ELN
Gene Alias
FLJ38671, FLJ43523, SVAS, WBS, WS
Gene Description
elastin
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that is one of the two components of elastic fibers. The encoded protein is rich in hydrophobic amino acids such as glycine and proline, which form mobile hydrophobic regions bounded by crosslinks between lysine residues. Deletions and mutations in this gene are associated with supravalvular aortic stenosis (SVAS) and autosomal dominant cutis laxa. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000196667|tropoelastin
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Interactome
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Disease
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Publication Reference
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Wound healing of human skin transplanted onto the nude mouse. II. An immunohistological and ultrastructural study of the epidermal basement membrane zone reconstruction and connective tissue reorganization.
Demarchez M, Hartmann DJ, Herbage D, Ville G, Prunieras M.
Developmental Biology 1987 May; 121(1):119.
Application:IF, IHC-Fr, Human, Mouse, Human skin, Mouse skin.
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Ultrastructural immunolocalization of elastic fibers in rat blood vessels using the protein A-gold technique.
Lethias C, Hartmann DJ, Masmejean M, Ravazzola M, Sabbagh I, Ville G, Herbage D, Eloy R.
The Journal of Histochemistry and Cytochemistry 1989 Jan; 35(1):15.
Application:IEM, IHC, RIA, Human, Rat, Human dermis, Rat aorta, Recombinant proteins.
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Wound healing of human skin transplanted onto the nude mouse. II. An immunohistological and ultrastructural study of the epidermal basement membrane zone reconstruction and connective tissue reorganization.
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