DDX3X polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of DDX3X.
Immunogen
A synthetic peptide corresponding to internal region 16 amino acids of human DDX3X.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
DDX3 antibody will detect both DDX3 and DBY.
Form
Liquid
Recommend Usage
Western Blot (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of DDX3X in HepG2 cell lysate with DDX3X polyclonal antibody (Cat # PAB13188) at (A) 1 and (B) 2 ug/mL .Immunocytochemistry
Immunocytochemistry of DDX3X in HepG2 cells with DDX3X polyclonal antibody (Cat # PAB13188) at 10 ug/mL . -
Gene Info — DDX3X
Entrez GeneID
1654Protein Accession#
AAC34298Gene Name
DDX3X
Gene Alias
DBX, DDX14, DDX3, HLP2
Gene Description
DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked
Omim ID
300160Gene Ontology
HyperlinkGene Summary
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which interacts specifically with hepatitis C virus core protein resulting a change in intracellular location. This gene has a homolog located in the nonrecombining region of the Y chromosome. The protein sequence is 91% identical between this gene and the Y-linked homolog. [provided by RefSeq
Other Designations
ATP-dependent RNA helicase DDX3X|CAP-Rf|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3|DEAD/H box-3|helicase like protein 2
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Interactome
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Disease
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Publication Reference
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RNA helicases: regulators of differentiation.
Abdelhaleem M.
Clinical Biochemistry 2005 Jun; 38(6):499.
Application:WB, Human, Human mammalian cells.
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Functional coherence of the human Y chromosome.
Lahn BT, Page DC.
Science 1997 Oct; 278(5338):675.
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Birth of the D-E-A-D box.
Linder P, Lasko PF, Ashburner M, Leroy P, Nielsen PJ, Nishi K, Schnier J, Slonimski PP.
Nature 1989 Jan; 337(6203):121.
Application:IP, WB-Ce, WB-Tr, Human, Mammalian cells.
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RNA helicases: regulators of differentiation.
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