BECN1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of BECN1 in A-431 cell lysate with BECN1 polyclonal antibody (Cat # PAB13167) at (A) 0.5, (B) 1 and (C) 2 ug/mL .
Immunocytochemistry
Immunocytochemistry staining of A-431 cells using BECN1 polyclonal antibody (Cat # PAB13167) at 1 ug/mL .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of BECN1.
Immunogen
A synthetic peptide corresponding to C-terminus 16 amino acids of human BECN1.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Recommend Usage
Western Blot (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for up to one year.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of BECN1 in A-431 cell lysate with BECN1 polyclonal antibody (Cat # PAB13167) at (A) 0.5, (B) 1 and (C) 2 ug/mL .Immunocytochemistry
Immunocytochemistry staining of A-431 cells using BECN1 polyclonal antibody (Cat # PAB13167) at 1 ug/mL . -
Gene Info — BECN1
Entrez GeneID
8678Protein Accession#
AAH10276Gene Name
BECN1
Gene Alias
ATG6, VPS30, beclin1
Gene Description
beclin 1, autophagy related
Omim ID
604378Gene Ontology
HyperlinkGene Summary
O
Other Designations
ATG6 autophagy related 6 homolog|beclin 1|beclin 1 (coiled-coil, moesin-like BCL2 interacting protein)|beclin 1 (coiled-coil, moesin-like BCL2-interacting protein)
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Interactome
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Pathway
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Disease
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Publication Reference
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Autophagy as a cell death and tumor suppressor mechanism.
Gozuacik D, Kimchi A.
Oncogene 2004 Apr; 23(16):2891.
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Apg14p and Apg6/Vps30p form a protein complex essential for autophagy in the yeast, Saccharomyces cerevisiae.
Kametaka S, Okano T, Ohsumi M, Ohsumi Y.
The Journal of Biological Chemistry 1998 Aug; 273(35):22284.
Application：WB-CE, WB-Tr, Yeast, Yeast cells.
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Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells.
Kisen GO, Tessitore L, Costelli P, Gordon PB, Schwarze PE, Baccino FM, Seglen PO.
Carcinogenesis 1993 Dec; 14(12):2501.
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Autophagy as a cell death and tumor suppressor mechanism.
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