BBC3 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of BBC3 expression in K-562 cell lysate with BBC3 polyclonal antibody (Cat # PAB13106) at 2 ug /mL .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of BBC3 in human breast carcinoma with BBC3 polyclonal antibody (Cat # PAB13106) at 10 ug/mL .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of BBC3.
Immunogen
A synthetic peptide corresponding to N-terminus 14 amino acids of human BBC3.
Host
Rabbit
Reactivity
Human
Specificity
This sequence is identical between a and b forms of the PUMA proteins. K-562 cell lysate can be used as a positive control and a band at approximately 23 KDa can be detected. antibody can also detect PUMA by immunohistochemistry at 10 ug/mL.
Form
Liquid
Recommend Usage
Western Blot (2-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of BBC3 expression in K-562 cell lysate with BBC3 polyclonal antibody (Cat # PAB13106) at 2 ug /mL .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of BBC3 in human breast carcinoma with BBC3 polyclonal antibody (Cat # PAB13106) at 10 ug/mL . -
Gene Info — BBC3
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Interactome
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Pathway
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Publication Reference
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Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals.
Han J, Flemington C, Houghton AB, Gu Z, Zambetti GP, Lutz RJ, Zhu L, Chittenden T.
PNAS 2001 Sep; 98(20):11318.
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PUMA, a novel proapoptotic gene, is induced by p53.
Nakano K, Vousden KH.
Molecular Cell 2001 Mar; 7(3):683.
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PUMA induces the rapid apoptosis of colorectal cancer cells.
Yu J, Zhang L, Hwang PM, Kinzler KW, Vogelstein B.
Molecular Cell 2001 Mar; 7(3):673.
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Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals.
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