ANP32E polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ANP32E.
Immunogen
A synthetic peptide corresponding to C-terminus of human ANP32E.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
A band at approximately 35 KDa can be detected. PHAP III has no cross-reaction to PHAP I and PHAP I2a.
Form
Liquid
Recommend Usage
Western Blot (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for up to one year.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of ANP32E expression in human A-549 (A) and HepG2 (B) cells, and rat testis (C) with ANP32E polyclonal antibody (Cat # PAB12900) at 1 ug/mL .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of ANP32E in human small intestine tissue with ANP32E polyclonal antibody (Cat # PAB12900) at 2 ug/mL . -
Gene Info — ANP32E
Entrez GeneID
81611Protein Accession#
NP_112182Gene Name
ANP32E
Gene Alias
LANP-L, LANPL, MGC5350
Gene Description
acidic (leucine-rich) nuclear phosphoprotein 32 family, member E
Omim ID
609611Gene Ontology
HyperlinkGene Summary
O
Other Designations
OTTHUMP00000014533|leucine-rich acidic nuclear protein like
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Interactome
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Publication Reference
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ANP32E induces tumorigenesis of triple-negative breast cancer cells by upregulating E2F1.
Xiong Z, Ye L, Zhenyu H, Li F, Xiong Y, Lin C, Wu X, Deng G, Shi W, Song L, Yuan Z, Wang X.
Molecular Oncology 2018 Jun; 12(6):896.
Application:WB-Ce, WB-Ti, Human, BT-474, SK-BR-3, MDA-MB-361, ZR-75-30, BT-549, MDA-MB-231, MDA-MB-468, SUM159PT cells, Breast cancer.
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Distinctive roles of PHAP proteins and prothymosin-alpha in a death regulatory pathway.
Jiang X, Kim HE, Shu H, Zhao Y, Zhang H, Kofron J, Donnelly J, Burns D, Ng SC, Rosenberg S, Wang X.
Science 2003 Jan; 299(5604):223.
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Apoptosis. Life and death decisions.
Nicholson DW, Thornberry NA.
Science 2003 Jan; 299(5604):214.
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ANP32E induces tumorigenesis of triple-negative breast cancer cells by upregulating E2F1.
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