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Last updated: 2014/9/15
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TNNT2 polyclonal antibody

  • Catalog # : PAB12744
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of TNNT2.
  • Immunogen:
  • A synthetic peptide corresponding to internal domain of human TNNT2.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 31
  • Reactivity:
  • Cat, Dog, Human, Mouse, Rabbit
  • Specificity:
  • This antibody recognizes 31 KDa of TNNT2 by Western blot.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • Western Blot (0.1-1 ug/mL)
    ELISA (0.01-0.1 ug/mL)
    Immunoprecipitation (2-5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.2 (10% Proclin300)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Tissue lysate from mouse heart was resolved onto 12% SDS-PAGE and transferred onto NC Membrane, then probed by TNNT2 polyclonal antibody (Cat # PAB12744) at 1 : 500, right lane. Left lane was depleted by immunizing peptide.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of human cardiac muscle stained with TNNT2 polyclonal antibody (Cat # PAB12744) at 1 : 50 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
  • Immunoprecipitation
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 7139
  • Gene Name:
  • TNNT2
  • Gene Alias:
  • CMH2,CMPD2,MGC3889,RCM3,TnTC,cTnT
  • Gene Description:
  • troponin T type 2 (cardiac)
  • Gene Summary:
  • The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000033864,OTTHUMP00000033867,cardiomyopathy, dilated 1D (autosomal dominant),cardiomyopathy, hypertrophic 2,troponin T type 2, cardiac,troponin T, cardiac muscle,troponin T2, cardiac
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