PTEN polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
The mouse brain tissue lysate resolved onto 10% SDS-PAGE, transferred onto NC membrane, and followed by an immunoblotting with PTEN polyclonal antibody (Cat # PAB12724) (Lane 1) at 1 : 500, or with a pre-incubation of immunizing peptide (Lane 2).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human prostate tissue stained with PTEN polyclonal antibody (Cat # PAB12724) at 1 : 100 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PTEN.
Immunogen
A synthetic peptide corresponding to C-terminus of human PTEN.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes human PTEN protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (1:100)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
The mouse brain tissue lysate resolved onto 10% SDS-PAGE, transferred onto NC membrane, and followed by an immunoblotting with PTEN polyclonal antibody (Cat # PAB12724) (Lane 1) at 1 : 500, or with a pre-incubation of immunizing peptide (Lane 2).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human prostate tissue stained with PTEN polyclonal antibody (Cat # PAB12724) at 1 : 100 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — PTEN
Entrez GeneID
5728Gene Name
PTEN
Gene Alias
10q23del, BZS, MGC11227, MHAM, MMAC1, PTEN1, TEP1
Gene Description
phosphatase and tensin homolog
Gene Ontology
HyperlinkGene Summary
This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. [provided by RefSeq
Other Designations
MMAC1 phosphatase and tensin homolog deleted on chromosome 10|OTTHUMP00000020032|mutated in multiple advanced cancers 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Genomic deletion of PTEN is associated with tumor progression and early PSA recurrence in ERG fusion-positive and fusion-negative prostate cancer.
Krohn A, Diedler T, Burkhardt L, Mayer PS, De Silva C, Meyer-Kornblum M, Kötschau D, Tennstedt P, Huang J, Gerhäuser C, Mader M, Kurtz S, Sirma H, Saad F, Steuber T, Graefen M, Plass C, Sauter G, Simon R, Minner S, Schlomm T.
The American Journal of Pathology 2012 Aug; 181(2):401.
Application：IHC, Human, Human prostate cancer.
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Cooperative phosphorylation of the tumor suppressor phosphatase and tensin homologue (PTEN) by casein kinases and glycogen synthase kinase 3beta.
Al-Khouri AM, Ma Y, Togo SH, Williams S, Mustelin T.
The Journal of Biological Chemistry 2005 Aug; 280(42):35195.
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Genomic deletion of PTEN is associated with tumor progression and early PSA recurrence in ERG fusion-positive and fusion-negative prostate cancer.
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