KRT19 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
The whole cell lysate derived from SK-BR-3 cell was immunoprobed by KRT19 polyclonal antibody (Cat # PAB12676) at 1:500. An immunoreactive band is observed around ~40kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse liver cell lysate was stained with KRT19 polyclonal antibody (Cat# PAB12676) at 1:500 for 10 min RT. Staining of formalinfixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of KRT19.
Immunogen
A synthetic peptide corresponding to N-terminus of human KRT19.
Host
Rabbit
Theoretical MW (kDa)
40
Reactivity
Human, Mouse
Specificity
This antibody only recognizes ~40 KDa of human KRT19.
Form
Liquid
Purification
Affinity Purification
Isotype
IgG
Recommend Usage
Flow Cytometry
ELISA (0.01-0.1 ug/mL)
Immunohistochemistry (0.5-2 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Western Blot (0.1-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (antibody stabilizer).
Storage Instruction
Store at 4°C. For long term storage store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
The whole cell lysate derived from SK-BR-3 cell was immunoprobed by KRT19 polyclonal antibody (Cat # PAB12676) at 1:500. An immunoreactive band is observed around ~40kDa.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse liver cell lysate was stained with KRT19 polyclonal antibody (Cat# PAB12676) at 1:500 for 10 min RT. Staining of formalinfixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
-
Gene Info — KRT19
Entrez GeneID
3880Gene Name
KRT19
Gene Alias
CK19, K19, K1CS, MGC15366
Gene Description
keratin 19
Omim ID
148020Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq
Other Designations
40-kDa keratin intermediate filament|cytokeratin 19|keratin, type I cytoskeletal 19|keratin, type I, 40-kd
-
Interactome
-
Disease
-
Publication Reference
-
Strengthened glycolysis under hypoxia supports tumor symbiosis and hexosamine biosynthesis in pancreatic adenocarcinoma.
Guillaumond F, Leca J, Olivares O, Lavaut MN, Vidal N, Berthezene P, Dusetti NJ, Loncle C, Calvo E, Turrini O, Iovanna JL, Tomasini R, Vasseur S.
PNAS 2013 Mar; 110(10):3919.
Application：IF, Mouse, Mouse pancreatic cancer cells.
-
Cytokeratin-19 associated with apoptosis and chemosensitivity in human cervical cancer cells.
Yuan CC, Huang HC, Tsai LC, Ng HT, Huang TS.
Apoptosis 1997 Jan; 2(1):101.
Application：Func, WB-Ce, Human, HeLa S3 cells.
-
Strengthened glycolysis under hypoxia supports tumor symbiosis and hexosamine biosynthesis in pancreatic adenocarcinoma.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com