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CTNNB1 polyclonal antibody   

  • Catalog # : PAB12658
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  • 100 ug
  • USD $ 319
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of CTNNB1.
  • Immunogen:
  • A synthetic peptide corresponding to residues surrounding S33 and S37 of human CTNNB1.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 88
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • This antibody recognizes ~88 KDa of human CTNNB1.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • Western Blot (0.1-1 ug/mL)
    ELISA (0.01-0.1 ug/mL)
    Immunoprecipitation (2-5 ug/mL)
    Immunohistochemistry (2-5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In TBS, pH 7.2 (10% Proclin300)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • The cell lysate derived from HeLa was immunoprobed at a dilution of 1 : 500 by the following antibodies.
    1 : Phospho-CTNNB1 S33/S37 polyclonal antibody (Cat # PAB12604).
    2 : Phospho-CTNNB1 S33 polyclonal antibody (Cat # PAB12603).
    3 : Phospho-CTNNB1 S37 polyclonal antibody (Cat # PAB12661).
    4 : CTNNB1 polyclonal antibody (Cat # PAB12658).
    5 : Negative control.
  • Immunohistochemistry
  • Immunoprecipitation
  • ELISA
  • Dot Blot (Peptide)
  • Dot Blot (Peptide)
  • Dot Blot : 1 ug peptide was blot onto NC membrane.
    A : CTNNB1 (pS33/S37).
    B : CTNNB1 (pS33).
    C : CTNNB1 (pS37).
    D : CTNNB1 (Non-phospho).
    E : Non-related Phospho-peptide.
    Followed were blotted at a 1 : 1000 dilution by :
    1 : Phospho-CTNNB1 S33/S37 polyclonal antibody (Cat # PAB12604).
    2: Phospho-CTNNB1 S33 polyclonal antibody (Cat # PAB12603).
    3 : Phospho-CTNNB1 S37 polyclonal antibody (Cat # PAB12661).
    4 : CTNNB1 polyclonal antibody (Cat # PAB12658).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 1499
  • Gene Name:
  • CTNNB1
  • Gene Alias:
  • CTNNB,DKFZp686D02253,FLJ25606,FLJ37923
  • Gene Description:
  • catenin (cadherin-associated protein), beta 1, 88kDa
  • Gene Summary:
  • Beta-catenin is an adherens junction protein. Adherens junctions (AJs; also called the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces. AJs mediate adhesion between cells, communicate a signal that neighboring cells are present, and anchor the actin cytoskeleton. In serving these roles, AJs regulate normal cell growth and behavior. At several stages of embryogenesis, wound healing, and tumor cell metastasis, cells form and leave epithelia. This process, which involves the disruption and reestablishment of epithelial cell-cell contacts, may be regulated by the disassembly and assembly of AJs. AJs may also function in the transmission of the 'contact inhibition' signal, which instructs cells to stop dividing once an epithelial sheet is complete.[supplied by OMIM
  • Other Designations:
  • OTTHUMP00000165222,OTTHUMP00000165223,catenin (cadherin-associated protein), beta 1 (88kD),catenin beta-1
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