Mycobacterium tuberculosis CFP10 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against Mycobacterium tuberculosis CFP10 (Rv3874).
Immunogen
CFP10 (Rv3874) of Mycobacterium tuberculosis.
Host
Rabbit
Theoretical MW (kDa)
12.3
Reactivity
Bacteria
Specificity
This antibody reacts with CFP10 (LHP), a 12.3 KDa antigen encoded by gene Rv3874 of Mycobacterium tuberculosis.
This antibody recognizes CFP10 of Mycobacterium tuberculosis H37Rv and Mycobacterium bovis; it reacts also with recombinant antigen produced in Escherichia coli.Form
Liquid
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blotting analysis (reducing conditions) of recombinant protein CFP10 using CFP10 polyclonal antibody (Cat # PAB1265). Lane 1 , 3 , 5 : Cell lysates of non - transfected E. coli (negative controls). Lane 2 , 4 , 6 : Cell lysate of CFP10 - transfected E. coli; dilution (2) 0.5 ug/mL , (4) 0.2 ug/mL , (6) 0.1 ug/mL ; detection with Donkey anti - rabbit / HRP secondary antibody.
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