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Last updated: 2014/7/28
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KDR polyclonal antibody

  • Catalog # : PAB12647
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of KDR.
  • Immunogen:
  • A synthetic peptide corresponding to extracellular domain of human KDR.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 200, 240
  • Reactivity:
  • Dog, Human, Mouse
  • Specificity:
  • This antibody recognizes ~200 and ~240 KDa of human KDR.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • Western Blot (0.1-1 ug/mL)
    ELISA (0.01-0.1 ug/mL)
    Immunoprecipitation (2-5 ug/mL)
    Immunohistochemistry (2-5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.2 (10% Proclin300)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Publication Reference
  • Applications
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of human breast cancer tissue was stained with KDR polyclonal antibody (Cat # PAB12647) at 1 : 100 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
  • Immunoprecipitation
  • Immunoprecipitation
  • The whole cell lysate derived from human umbilical vein endothelial cell (HUVEC) was stimulated by VEGF-A for 20 min, then immunoprecipitated by KDR polyclonal antibody (Cat # PAB12647) followed by immune-probing with KDR polyclonal antibody (Cat # PAB12647) at 1 : 500. The immunoreactive bands are observed around ~ 200 KDa (non-phospho form) and ~ 240 KDa (Phospho-form, lane 1). The lane 2 is a negative control.
  • ELISA
  • Application Image
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 3791
  • Gene Name:
  • KDR
  • Gene Alias:
  • CD309,FLK1,VEGFR,VEGFR2
  • Gene Description:
  • kinase insert domain receptor (a type III receptor tyrosine kinase)
  • Gene Summary:
  • Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq
  • Other Designations:
  • soluble VEGFR2,vascular endothelial growth factor receptor 2
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