Immunohistochemical staining of human breast cancer tissue was stained with KDR polyclonal antibody (Cat # PAB12647) at 1 : 100 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
The whole cell lysate derived from human umbilical vein endothelial cell (HUVEC) was stimulated by VEGF-A for 20 min, then immunoprecipitated by KDR polyclonal antibody (Cat # PAB12647) followed by immune-probing with KDR polyclonal antibody (Cat # PAB12647) at 1 : 500. The immunoreactive bands are observed around ~ 200 KDa (non-phospho form) and ~ 240 KDa (Phospho-form, lane 1). The lane 2 is a negative control.
Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq