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TP53 (phospho S15) polyclonal antibody   

  • Catalog # : PAB12640
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  • 100 ug
  • USD $ 335
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of TP53.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding S15 of human TP53.
  • Sequence:
  • PLSQE
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 53
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • This antibody recognizes ~53 KDa of human TP53. It does not recognize non-phosphorylated peptides.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • Western Blot (0.1-1 ug/mL)
    ELISA (0.01-0.1 ug/mL)
    Immunoprecipitation (2-5 ug/mL)
    Immunohistochemistry (2-5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.2 (10% Proclin300)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • The cell lysate derived from UV stimulated HeLa cells was immunoprobed by TP53 (phospho S15) polyclonal antibody (Cat # PAB12640) at 1 : 500. An immunoreactive band is observed around ~ 53 KDa (Lane 1). The lane 2 is a negative control.
  • Immunocytochemistry
  • Immunocytochemistry
  • Immunocytochemical staining of UV treated HeLa cells cultured in the cell chamber, fixed, and immunoprobed by TP53 (phospho S15) polyclonal antibody (Cat # PAB12640) at 1 : 100 for 10 min at RT.
  • Immunoprecipitation
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 7157
  • Gene Name:
  • TP53
  • Gene Alias:
  • FLJ92943,LFS1,TRP53,p53
  • Gene Description:
  • tumor protein p53
  • Gene Summary:
  • This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity. [provided by RefSeq
  • Other Designations:
  • p53 antigen,p53 transformation suppressor,p53 tumor suppressor,phosphoprotein p53,transformation-related protein 53
  • Interactome
  • Related Disease
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