NF1 (phospho S2741) polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
The tissue lysates derived from hypoxic mouse brain were immunoblotted by NF1 (phospho S2741) polyclonal antibody (Cat # PAB12637) at 1 : 1000.
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane.
A : NF1 (pS2741).
B : NF1 (Non-phospho).
C : Non-related phospho-specific phophopeptide.
Followed were blotted at a 1 : 1000 dilution by :
1. NF1 (phospho S2741) polyclonal antibody (Cat # PAB12637).
2. NF1 polyclonal antibody (Cat # PAB12636).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of NF1.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S2741 of human NF1.
Sequence
PTSPY
Host
Rabbit
Theoretical MW (kDa)
240
Reactivity
Bovine, Dog, Human, Mouse, Rat
Specificity
This antibody recognizes ~240 KDa of human NF1 at the phosphorylation site of Ser 2741. It does not cross react to non-phospho NF1.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
The tissue lysates derived from hypoxic mouse brain were immunoblotted by NF1 (phospho S2741) polyclonal antibody (Cat # PAB12637) at 1 : 1000.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane.
A : NF1 (pS2741).
B : NF1 (Non-phospho).
C : Non-related phospho-specific phophopeptide.
Followed were blotted at a 1 : 1000 dilution by :
1. NF1 (phospho S2741) polyclonal antibody (Cat # PAB12637).
2. NF1 polyclonal antibody (Cat # PAB12636). -
Gene Info — NF1
Entrez GeneID
4763Gene Name
NF1
Gene Alias
DKFZp686J1293, FLJ21220, NFNS, VRNF, WSS
Gene Description
neurofibromin 1
Gene Ontology
HyperlinkGene Summary
This gene product appears to function as a negative regulator of the ras signal transduction pathway. Mutations in this gene have been linked to neurofibromatosis type 1, juvenile myelomonocytic leukemia and Watson syndrome. The mRNA for this gene is subject to RNA editing (CGA>UGA->Arg1306Term) resulting in premature translation termination. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene. [provided by RefSeq
Other Designations
neurofibromin
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Interactome
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Pathway
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Disease
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Publication Reference
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Phosphorylation of neurofibromin by cAMP-dependent protein kinase is regulated via a cellular association of N(G),N(G)-dimethylarginine dimethylaminohydrolase.
Tokuo H, Yunoue S, Feng L, Kimoto M, Tsuji H, Ono T, Saya H, Araki N.
FEBS Letters 2001 Apr; 494(1-2):48.
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Phosphorylation of neurofibromin by cAMP-dependent protein kinase is regulated via a cellular association of N(G),N(G)-dimethylarginine dimethylaminohydrolase.
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