BCL2 (phospho T129) polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of BCL2.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding T129 of human BCL2.
Sequence
FATVV
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes BCL2 with the phosphorylation site Thr129. It does not cross-react with non-phosphospecific peptide.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (2-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
The whole cell lysate derived from UV treated Raji cell was separated in 10% SDS-PAGE, transferred onto NC membrane, and immunoblotted by BCL2 (phospho T129) polyclonal antibody (Cat # PAB12608) at 1 : 500. An immunoreactive band around ~ 26 kDa is observed.Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — BCL2
Entrez GeneID
596Gene Name
BCL2
Gene Alias
Bcl-2
Gene Description
B-cell CLL/lymphoma 2
Omim ID
151430Gene Ontology
HyperlinkGene Summary
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq
Other Designations
B-cell lymphoma protein 2|OTTHUMP00000163680
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Interactome
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Pathway
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Disease
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Publication Reference
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Statistical interpretation of CA125 and Bcl-2 in serum of patients with late stage ovarian cancer.
Camlica H, Duranyildiz D, Tas F, Yasasever V.
American Journal of Clinical Oncology 2008 Dec; 31(6):585.
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Statistical interpretation of CA125 and Bcl-2 in serum of patients with late stage ovarian cancer.
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