BBC3 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of BBC3.
Immunogen
A synthetic peptide corresponding to exon-3-encoded region of human BBC3.
Sequence
EQHLESPVPSAPGALAG
Host
Rabbit
Reactivity
Human
Specificity
Reacts with a 17 residue sequence [EQHLESPVPSAPGALAG] found in the exon-3-encoded region in the human PUMA-alpha and PUMA-beta forms of the protein. Recognizes both forms of the protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:500-1:1)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
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Gene Info — BBC3
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Interactome
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Pathway
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Publication Reference
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Aberrant activation of notch signaling in human breast cancer.
Stylianou S, Clarke RB, Brennan K.
Cancer Research 2006 Feb; 66(3):1517.
Application:WB-Tr, Human, MCF-10A cells.
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PUMA, a novel proapoptotic gene, is induced by p53.
Nakano K, Vousden KH.
Molecular Cell 2001 Mar; 7(3):683.
Application:IP-WB, WB-Tr, Human, NCI-H1299, U-2 OS cells.
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Aberrant activation of notch signaling in human breast cancer.
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