VEGFA polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of VEGFA.
Immunogen
A synthetic peptide corresponding to amino acids at N-terminus of human VEGFA.
Host
Rabbit
Theoretical MW (kDa)
27
Reactivity
Human, Mouse, Rat
Specificity
Identical to the related rat sequence.
Form
Lyophilized
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Western Blot (0.5-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.9 mg NaCl, 0.2 mg Na2HPO4 (5 mg BSA, 0.05 mg sodium azide, 0.05 mg Thimerosal)
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 200 uL of deionized water and concentration will be 500 ug/mL, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide and thimerosal: POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western Blot analysis of VEGFA expression from rat cardiac muscle tissue lyate with VEGFA polyclonal antibody (Cat # PAB12284).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of VEGFA on formalin fixed, paraffin embedded human esophageal squamous cell carcinoma (ESCC) in cytoplasm DAB chromogenic reaction. Using VEGFA polyclonal antibody (Cat # PAB12284). -
Gene Info — VEGFA
Entrez GeneID
7422GeneBank Accession#
BC065522Protein Accession#
AAH65522Gene Name
VEGFA
Gene Alias
MGC70609, VEGF, VEGF-A, VPF
Gene Description
vascular endothelial growth factor A
Gene Ontology
HyperlinkGene Summary
This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq
Other Designations
vascular endothelial growth factor isoform VEGF165|vascular permeability factor
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Interactome
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Pathway
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Disease
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Publication Reference
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Protective effect of Salvianolic acid A on ischaemia-reperfusion acute kidney injury in rats through protecting against peritubular capillary endothelium damages.
Zhang Z, Qi D, Wang X, Gao Z, Li P, Liu W, Tian X, Liu Y, Yang M, Liu K, Fan H.
Phytotherapy Research 2017 Oct; 32(1):103.
Application:IHC-P, Rat, Rat kidney.
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Opposing roles of CXCR4 and CXCR7 in breast cancer metastasis.
Hernandez L, Magalhaes MA, Coniglio SJ, Condeelis JS, Segall JE.
Breast Cancer Research 2011 Dec; 13(6):R128.
Application:IHC-P, Mouse, Mouse tumors.
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Vascular endothelial growth factor (VEGF) induces remodeling and enhances TH2-mediated sensitization and inflammation in the lung.
Lee CG, Link H, Baluk P, Homer RJ, Chapoval S, Bhandari V, Kang MJ, Cohn L, Kim YK, McDonald DM, Elias JA.
Nature Medicine 2004 Oct; 10(10):1095.
Application:ELISA, IF, IHC, Mouse, Lung, T cells.
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Vascular endothelial growth factor (VEGF) stimulates neurogenesis in vitro and in vivo.
Jin K, Zhu Y, Sun Y, Mao XO, Xie L, Greenberg DA.
PNAS 2002 Sep; 99(18):11946.
Application:Inject, Recombinant protein.
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VEGF regulates haematopoietic stem cell survival by an internal autocrine loop mechanism.
Gerber HP, Malik AK, Solar GP, Sherman D, Liang XH, Meng G, Hong K, Marsters JC, Ferrara N.
Nature 2002 Jun; 417(6892):954.
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Protective effect of Salvianolic acid A on ischaemia-reperfusion acute kidney injury in rats through protecting against peritubular capillary endothelium damages.
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