Rabbit polyclonal antibody raised against synthetic peptide of SOX2.
Immunogen:
A synthetic peptide corresponding to amino acids 1-100 of human SOX2.
Host:
Rabbit
Reactivity:
Chicken, Human, Mouse, Sheep, Xenopus, Zebra fish
Form:
Liquid
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
Recommend Usage:
Flow Cytometry (1:100) Immunocytochemistry (1:125-1:250) Immunofluorescence (1:50-1:200) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:125-1:250) Western Blot (0.5 ug/mL) The optimal working dilution should be determined by the end user.
Storage Buffer:
In Tris-glycine, 150 mM NaCl (0.05% sodium azide)
Storage Instruction:
Store at -20°C or -80°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Applications
Western Blot (Tissue lysate)
Western blot analysis of SOX2 in mouse brain lysate with SOX2 polyclonal antibody at 0.5 ug/mL (Cat # PAB12025).
This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq
Other Designations:
SRY-related HMG-box gene 2,sex-determining region Y-box 2,transcription factor SOX2