EPM2A polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
EPM2A polyclonal antibody (Cat # PAB11469) (0.1 ug/mL) staining of human cerebellum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
EPM2A polyclonal antibody (Cat # PAB11469) (3.8 ug/mL) staining of paraffin embedded human cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of EPM2A.
Immunogen
A synthetic peptide corresponding to internal region of human EPM2A.
Sequence
C-EATGHTNEMKHTTD
Host
Goat
Theoretical MW (kDa)
37.2, 35.5
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Western Blot (0.1-0.3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2.5-3.8 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
EPM2A polyclonal antibody (Cat # PAB11469) (0.1 ug/mL) staining of human cerebellum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
EPM2A polyclonal antibody (Cat # PAB11469) (3.8 ug/mL) staining of paraffin embedded human cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — EPM2A
Entrez GeneID
7957Protein Accession#
NP_005661.1Gene Name
EPM2A
Gene Alias
EPM2, MELF
Gene Description
epilepsy, progressive myoclonus type 2A, Lafora disease (laforin)
Gene Ontology
HyperlinkGene Summary
This gene encodes a dual-specificity phosphatase that associates with polyribosomes. The encoded protein may be involved in the regulation of glycogen metabolism. Mutations in this gene have been associated with myoclonic epilepsy of Lafora. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
OTTHUMP00000017360|epilepsy, progressive myoclonus type 2, Lafora disease (laforin)|laforin
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Interactome
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Disease
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Publication Reference
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Lafora disease proteins malin and laforin are recruited to aggresomes in response to proteasomal impairment.
Mittal S, Dubey D, Yamakawa K, Ganesh S.
Human Molecular Genetics 2007 Apr; 16(7):753.
Application：IF, Human, Monkey, COS-7, HEK 293 cells.
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Lafora disease proteins malin and laforin are recruited to aggresomes in response to proteasomal impairment.
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