S100A1/S100B polyclonal antibody
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More Files
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Western Blot
Western blot using S100A1/S100B polyclonal antibody (Cat # PAB11341) shows detection of a band ~11 KDa corresponding to bovine S100A1/S100B monomer (100 ng loaded, arrowhead lane 1).
The antibody also detects S100A1/S100B from rat brain lysate (Lane 2). Approximately 35 ug of a rat brain whole cell lysate was separated by 16% SDS-PAGE and transferred onto nitrocellulose.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of S100A1/S100B polyclonal antibody (Cat # PAB11341) was used at a 1:500 dilution to detect S100A1/S100B by immunohistochemistry in human brain astrocyte tumor tissue.
Tissue was formalin-fixed and paraffin embedded.
Personal Communication,Alan Yen, Life Span Biosciences, Seattle, WA.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of S100A1/S100B polyclonal antibody (Cat # PAB11341) was used at a 1:500 dilution to detect S100A1/S100B.
Dark nuclear staining is observed within basket cells located near the Purkinje cells in the mouse cerebellum.
Personal Communication, Teri Johnson, Stowers Institute, Kansas City, MO.
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant S100A1/S100B.
Immunogen
Recombinant protein corresponding to full length bovine S100A1/S100B.
Host
Rabbit
Reactivity
Bovine, Dog, Mouse, Rat
Specificity
A 98% homology is noted for S100 alpha chain from primate sources. Mouse, rat and dog show 94% homology with the bovine S100 alpha sequence. Expect cross reactivity with S100 from most mammalian sources. Homologies for the S100 beta chain are similar.
Form
Lyophilized
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:5000-1:25000)
Western Blot (1:500-1:3000)
Immunohistochemistry (1:200-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot after reconstitution to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot using S100A1/S100B polyclonal antibody (Cat # PAB11341) shows detection of a band ~11 KDa corresponding to bovine S100A1/S100B monomer (100 ng loaded, arrowhead lane 1).
The antibody also detects S100A1/S100B from rat brain lysate (Lane 2). Approximately 35 ug of a rat brain whole cell lysate was separated by 16% SDS-PAGE and transferred onto nitrocellulose.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of S100A1/S100B polyclonal antibody (Cat # PAB11341) was used at a 1:500 dilution to detect S100A1/S100B by immunohistochemistry in human brain astrocyte tumor tissue.
Tissue was formalin-fixed and paraffin embedded.
Personal Communication,Alan Yen, Life Span Biosciences, Seattle, WA.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of S100A1/S100B polyclonal antibody (Cat # PAB11341) was used at a 1:500 dilution to detect S100A1/S100B.
Dark nuclear staining is observed within basket cells located near the Purkinje cells in the mouse cerebellum.
Personal Communication, Teri Johnson, Stowers Institute, Kansas City, MO.Enzyme-linked Immunoabsorbent Assay
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Gene Info — S100B
Entrez GeneID
525716Protein Accession#
P02639 (Bovine gene ID : 528735)Gene Name
S100B
Gene Alias
MGC128275
Gene Description
S100 calcium binding protein B
Gene Ontology
HyperlinkGene Summary
beta polypeptide
Other Designations
S100 protein, beta polypeptide
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Gene Info — S100A1
Entrez GeneID
528735Protein Accession#
P02639 (Bovine gene ID : 528735)Gene Name
S100A1
Gene Alias
MGC159706
Gene Description
S100 calcium binding protein A1
Gene Ontology
HyperlinkOther Designations
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Publication Reference
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Acidic nanoparticles protect against α-synuclein-induced neurodegeneration through the restoration of lysosomal function.
Marie-Laure Arotcarena, Federico N Soria, Anthony Cunha, Evelyne Doudnikoff, Geoffrey Prévot, Jonathan Daniel, Mireille Blanchard-Desce, Philippe Barthélémy, Erwan Bezard, Sylvie Crauste-Manciet, Benjamin Dehay.
Aging Cell 2022 Apr; 21(4):e13584.
Application：IHC, Mouse, Striatum, Substantia nigra.
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Brain injections of glial cytoplasmic inclusions induce a multiple system atrophy-like pathology.
Margaux Teil, Sandra Dovero, Mathieu Bourdenx, Marie-Laure Arotcarena, Sandrine Camus, Gregory Porras, Marie-Laure Thiolat, Ines Trigo-Damas, Celine Perier, Cristina Estrada, Nuria Garcia-Carrillo, Michele Morari, Wassilios G Meissner, María Trinidad Herrero, Miquel Vila, Jose A Obeso, Erwan Bezard, Benjamin Dehay.
Brain 2022 Apr; 145(3):1001.
Application：IHC-Fr, Baboon, Baboon brain striatal.
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Transcription factor EB overexpression prevents neurodegeneration in experimental synucleinopathies.
Arotcarena ML, Bourdenx M, Dutheil N, Thiolat ML, Doudnikoff E, Dovero S, Ballabio A, Fernagut PO, Meissner WG, Bezard E, Dehay B.
JCI Insight 2019 Aug; 4(16):129719.
Application：IHC-P, Mouse, Mouse striatal sections.
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Astrocytosis in parkinsonism: considering tripartite striatal synapses in physiopathology?
Charron G, Doudnikoff E, Canron MH, Li Q, Véga C, Marais S, Baufreton J, Vital A, Oliet SH, Bezard E.
Frontiers in Aging Neuroscience 2014 Sep; 6:258.
Application：IHC, Mouse, Rat, Brain.
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Endogenous morphine-like compound immunoreactivity increases in parkinsonism.
Charron G, Doudnikoff E, Laux A, Berthet A, Porras G, Canron MH, Barroso-Chinea P, Li Q, Qin C, Nosten-Bertrand M, Giros B, Delalande F, Van Dorsselaer A, Vital A, Goumon Y, Bezard E.
Brain 2011 Aug; 134(Pt 8):2321.
Application：IF, Rat, Brain.
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S100 beta is a target protein of neurocalcin delta, an abundant isoform in glial cells.
Okazaki K, Obata NH, Inoue S, Hidaka H.
The Biochemical Journal 1995 Mar; 306 ( Pt 2)(Pt 2):551.
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Molecular cloning of cDNA of S100 alpha subunit mRNA.
Kuwano R, Maeda T, Usui H, Araki K, Yamakuni T, Ohshima Y, Kurihara T, Takahashi Y.
FEBS Letters 1986 Jun; 202(1):97.
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Ions binding to S100 proteins: structural changes induced by calcium and zinc on S100a and S100b proteins.
Baudier J, Gerard D.
Biochemistry 1983 Jul; 22(14):3360.
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Acidic nanoparticles protect against α-synuclein-induced neurodegeneration through the restoration of lysosomal function.
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