Rabbit polyclonal antibody raised against synthetic phosphopeptide of SMAD3.
Synthetic phosphopeptide corresponding to residues surrounding S423/S425 of human SMAD3.
Bovine, Chicken, Clawed frog, Frog, Human, Mouse, Pig, Rat, Zebra fish
Reactivity occurs against human Smad3 pS423 and pS425 protein and This antibody is specific to the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad1 and Smad5. Negligible reactivity is seen against other phosphorylated Smad family members.
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
ELISA (1:75000-1:300000) Western Blot (1:2000-1:20000) Immunohistochemistry (1:500) The optimal working dilution should be determined by the end user.
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot using SMAD3 (phospho S423/425) polyclonal antibody (Cat # PAB11304) shows detection of endogenous SMAD3 in stimulated cell lysates. Lysates were prepared from control cells (- lanes), or cells stimulated with 2 ng/mL TGF-beta (transforming growth factor) for 1 hour. This reagent recognizes phosphorylated Smad3 and has negligible reactivity against non-phosphorylated SMAD3 protein. Personal Communication. Ying Zhang, NIH, CCR, Bethesda, MD.
Immunohistochemical staining of SMAD3 (phospho S423/425) polyclonal antibody (Cat # PAB11304) was used at 2.5 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows strong nuclear staining in the majority of epidermal keratinocytes at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communi-cation, Tina Roush, LifeSpanBiosciences, Seattle, WA.
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq
MAD, mothers against decapentaplegic homolog 3,SMA- and MAD-related protein 3,SMAD, mothers against DPP homolog 3,mad homolog JV15-2,mad protein homolog,mothers against decapentaplegic homolog 3