Rabbit polyclonal antibody raised against synthetic peptide of SMAD3.
A synthetic peptide corresponding to amino acids 417-425 of human SMAD3.
Bovine, Chicken, Clawed frog, Frog, Human, Mouse, Pig, Rat, Zebra fish
ELISA and western blot show equivalent reactivity against phosphorylated and non-phosphorylated human Smad3. Expect reactivity against Smad1 and Smad5. Negligible reactivity is seen against other Smad family members.
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
ELISA (1:10000-1:40000) Western Blot (1:500-1:3000) Immunohistochemistry (1:500-1:3000) The optimal working dilution should be determined by the end user.
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot using SMAD3 polyclonal antibody (Cat # PAB11263) shows detection of endogenous SMAD3 in both unstimulated and stimulated cell lysates. Lysates were prepared from control cells (- lanes), or cells stimulated with 2 ng/mL TGF-beta (+ lanes) for 1 hour. This reagent recognizes both non-phosphorylated and phosphorylated SMAD3 protein. Personal Communication, Ying Zhang, NIH, CCR, Bethesda, MD.
Immunohistochemical staining with SMAD3 polyclonal antibody (Cat # PAB11263) shows strong cytoplasmic and membranous staining of tumor cells in cancerous human liver tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei. Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq
MAD, mothers against decapentaplegic homolog 3,SMA- and MAD-related protein 3,SMAD, mothers against DPP homolog 3,mad homolog JV15-2,mad protein homolog,mothers against decapentaplegic homolog 3