SMAD3 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot using SMAD3 polyclonal antibody (Cat # PAB11263) shows detection of endogenous SMAD3 in both unstimulated and stimulated cell lysates.
Lysates were prepared from control cells (- lanes), or cells stimulated with 2 ng/mL TGF-beta (+ lanes) for 1 hour.
This reagent recognizes both non-phosphorylated and phosphorylated SMAD3 protein.
Personal Communication, Ying Zhang, NIH, CCR, Bethesda, MD.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining with SMAD3 polyclonal antibody (Cat # PAB11263) shows strong cytoplasmic and membranous staining of tumor cells in cancerous human liver tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei.
Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SMAD3.
Immunogen
A synthetic peptide corresponding to amino acids 417-425 of human SMAD3.
Host
Rabbit
Reactivity
Bovine, Chicken, Clawed frog, Frog, Human, Mouse, Pig, Rat, Zebra fish
Specificity
ELISA and western blot show equivalent reactivity against phosphorylated and non-phosphorylated human Smad3. Expect reactivity against Smad1 and Smad5. Negligible reactivity is seen against other Smad family members.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:10000-1:40000)
Western Blot (1:500-1:3000)
Immunohistochemistry (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using SMAD3 polyclonal antibody (Cat # PAB11263) shows detection of endogenous SMAD3 in both unstimulated and stimulated cell lysates.
Lysates were prepared from control cells (- lanes), or cells stimulated with 2 ng/mL TGF-beta (+ lanes) for 1 hour.
This reagent recognizes both non-phosphorylated and phosphorylated SMAD3 protein.
Personal Communication, Ying Zhang, NIH, CCR, Bethesda, MD.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining with SMAD3 polyclonal antibody (Cat # PAB11263) shows strong cytoplasmic and membranous staining of tumor cells in cancerous human liver tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei.
Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SMAD3
Entrez GeneID
4088Protein Accession#
NP_005893;P84022Gene Name
SMAD3
Gene Alias
DKFZp586N0721, DKFZp686J10186, HSPC193, HsT17436, JV15-2, MADH3, MGC60396
Gene Description
SMAD family member 3
Omim ID
603109Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq
Other Designations
MAD, mothers against decapentaplegic homolog 3|SMA- and MAD-related protein 3|SMAD, mothers against DPP homolog 3|mad homolog JV15-2|mad protein homolog|mothers against decapentaplegic homolog 3
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Interactome
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Pathway
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Disease
- Alzheimer disease
- Anemia
- Asthma
- Bacteremia
- Breast cancer
- Breast Neoplasms
- Cleft Lip
- Cleft Palate
- Coronary Artery Disease
+ View More Disease
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Publication Reference
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Smad-dependent and Smad-independent pathways in TGF-beta family signalling.
Derynck R, Zhang YE.
Nature 2003 Oct; 425(6958):577.
Application：WB-Tr, Human, Mammalian cells.
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Mechanisms of TGF-beta signaling from cell membrane to the nucleus.
Shi Y, Massagué J.
Cell 2003 Jun; 113(6):685.
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Receptor-associated Mad homologues synergize as effectors of the TGF-beta response.
Zhang Y, Feng X, We R, Derynck R.
Nature 1996 Sep; 383(6596):168.
Application：IP, WB-Re, WB-Tr, Human, HEK 293 cells, Recombinant proteins.
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Smad-dependent and Smad-independent pathways in TGF-beta family signalling.
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