PRKDC (phospho T2609) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot using PRKDC (phospho T2609) polyclonal antibody (Cat # PAB10324) shows detection of a 460 KDa band corresponding to human PRKDC in various preparations.
Lane 1 : Fus1 untreated, Lane 2 : Fus1 IR (20Gy, 4h), Lane 3 : Fus1 PRKDC inhibitor + IR, Lane 4 : MO59J (DNAPK-) untreated, Lane 5 : MO59J IR, Lane 6 : Fus1 untreated, Lane 7 : Fus1 IR (20Gy, 4h), Lane 8 : Fus1 DNAPK inhibitor + IR, Lane 9 : MO59J untreated, Lane 10 : MO59J IR.
Lanes 1~5 are nuclear extract whereas lanes 6~10 are whole cell lysates.
MO59J is a cell line that lacks PRKDC.
FUS1 is the matched cell line complemented with a chromosomal fragment containing the PRKDC gene.
Approximately 20 $micro;g of lysate was run on SDS-PAGE and transferred onto nitrocellulose, followed by reaction with a 1 : 1,000 dilution of PRKDC (phospho T2609) polyclonal antibody (Cat # PAB10324).
Detection occurred using a 1 : 5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature.
A chemiluminescence system was used for signal detection using a 1 min exposure time.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of PRKDC.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding T2609 of human PRKDC.
Host
Rabbit
Reactivity
Chicken, Chimpanzee, Dog, Horse, Human, Rat
Specificity
This phospho-specific polyclonal antibody reacts with phosphorylated pT2609 of human DNA PKcs. Reactivity with non-phosphorylated human DNA PKcs is minimal.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:300000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using PRKDC (phospho T2609) polyclonal antibody (Cat # PAB10324) shows detection of a 460 KDa band corresponding to human PRKDC in various preparations.
Lane 1 : Fus1 untreated, Lane 2 : Fus1 IR (20Gy, 4h), Lane 3 : Fus1 PRKDC inhibitor + IR, Lane 4 : MO59J (DNAPK-) untreated, Lane 5 : MO59J IR, Lane 6 : Fus1 untreated, Lane 7 : Fus1 IR (20Gy, 4h), Lane 8 : Fus1 DNAPK inhibitor + IR, Lane 9 : MO59J untreated, Lane 10 : MO59J IR.
Lanes 1~5 are nuclear extract whereas lanes 6~10 are whole cell lysates.
MO59J is a cell line that lacks PRKDC.
FUS1 is the matched cell line complemented with a chromosomal fragment containing the PRKDC gene.
Approximately 20 $micro;g of lysate was run on SDS-PAGE and transferred onto nitrocellulose, followed by reaction with a 1 : 1,000 dilution of PRKDC (phospho T2609) polyclonal antibody (Cat # PAB10324).
Detection occurred using a 1 : 5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature.
A chemiluminescence system was used for signal detection using a 1 min exposure time.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PRKDC
Entrez GeneID
5591Protein Accession#
P78527;NP_008835Gene Name
PRKDC
Gene Alias
DNA-PKcs, DNAPK, DNPK1, HYRC, HYRC1, XRCC7, p350
Gene Description
protein kinase, DNA-activated, catalytic polypeptide
Omim ID
600899Gene Ontology
HyperlinkGene Summary
The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties.[supplied by OMIM
Other Designations
hyper-radiosensitivity of murine scid mutation, complementing 1
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Interactome
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Pathway
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Disease
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Publication Reference
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GSK3β negatively regulates TRAX, a scaffold protein implicated in mental disorders, for NHEJ-mediated DNA repair in neurons.
Chien T, Weng YT, Chang SY, Lai HL, Chiu FL, Kuo HC, Chuang DM, Chern Y.
Molecular Psychiatry 2018 Jan; [Epub].
Application：WB, Rat, PC-12 cells.
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Proteolytic cleavage of the catalytic subunit of DNA-dependent protein kinase during poliovirus infection.
Graham KL, Gustin KE, Rivera C, Kuyumcu-Martinez NM, Choe SS, Lloyd RE, Sarnow P, Utz PJ.
Journal of Virology 2004 Jun; 78(12):6313.
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Ku70/Ku80 and DNA-dependent protein kinase catalytic subunit modulate RAG-mediated cleavage: implications for the enforcement of the 12/23 rule.
Sawchuk DJ, Mansilla-Soto J, Alarcon C, Singha NC, Langen H, Bianchi ME, Lees-Miller SP, Nussenzweig MC, Cortes P.
The Journal of Biological Chemistry 2004 Jul; 279(28):29821.
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ATM and the catalytic subunit of DNA-dependent protein kinase activate NF-kappaB through a common MEK/extracellular signal-regulated kinase/p90(rsk) signaling pathway in response to distinct forms of DNA damage.
Panta GR, Kaur S, Cavin LG, Cortés ML, Mercurio F, Lothstein L, Sweatman TW, Israel M, Arsura M.
Molecular and Cellular Biology 2004 Mar; 24(5):1823.
Application：WB, Mouse, CHO cells.
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GSK3β negatively regulates TRAX, a scaffold protein implicated in mental disorders, for NHEJ-mediated DNA repair in neurons.
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