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Last updated: 2014/7/21
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PCNA polyclonal antibody

  • Catalog # : PAB10307
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of PCNA.
  • Immunogen:
  • A synthetic peptide corresponding to internal region of human PCNA.
  • Host:
  • Rabbit
  • Reactivity:
  • Bovine, Chicken, Clawed frog, Dog, Human, Monkey, Mouse, Rat
  • Specificity:
  • This affinity purified antibody is directed against human PCNA protein.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:2000-1:8000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using PCNA polyclonal antibody (Cat # PAB10307) shows detection of PCNA protein in HEK293 (Lane 1) and Jurkat (Lane 2) whole cell extracts.
    Approximately 25 ug of lysate was loaded per lane onto a 4-20% gradient gel followed by transfer to nitrocellulose.
    After blocking, the membrane was incubated with the primary antibody diluted to 1 : 1000.
    The membrane was washed and reacted with a 1 : 10,000 dilution of IRDye® 800 Conjugated Affinity Purified Goat-anti-Rabbit IgG [H&L] MX10 (800 nm channel, green).
    Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (Lane M, 700 nm channel, red).
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 5111
  • Protein Accession#:
  • NP_872590;P12004
  • Gene Name:
  • PCNA
  • Gene Alias:
  • MGC8367
  • Gene Description:
  • proliferating cell nuclear antigen
  • Gene Summary:
  • The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq
  • Other Designations:
  • DNA polymerase delta auxiliary protein,OTTHUMP00000030189,OTTHUMP00000030190,cyclin
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