POU5F1 polyclonal antibody
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More Files
- More Functions
Western Blot
Western blot using POU5F1 polyclonal antibody (Cat # PAB10296) shows detection of endogenous POU5F1 in mouse embryonic fibroblast (MEF) cell lysate (lane 1) and HeLa nuclear extract (HN) (lane 2).
The band at ~39 kDa (arrowhead) corresponds to POU5F1.
After transfer, the membrane was blocked with 5% BSA.
Primary antibody was used at a 1 : 1,000 dilution in PBS containing 5% BSA.
The specificity of the antibody was confirmed by peptide competition which blocks reaction of the antibody with POU5F1 (lanes 3 and 4, respectively).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of POU5F1.
Immunogen
A synthetic peptide corresponding to internal region of human POU5F1.
Host
Rabbit
Reactivity
Bovine, Chimpanzee, Dog, Horse, Human, Macaque, Mink, Mouse, Pig
Specificity
This antibody reacts with human and mouse Oct-4 protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:350000)
Western Blot (1:1000-1:1500)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot using POU5F1 polyclonal antibody (Cat # PAB10296) shows detection of endogenous POU5F1 in mouse embryonic fibroblast (MEF) cell lysate (lane 1) and HeLa nuclear extract (HN) (lane 2).
The band at ~39 kDa (arrowhead) corresponds to POU5F1.
After transfer, the membrane was blocked with 5% BSA.
Primary antibody was used at a 1 : 1,000 dilution in PBS containing 5% BSA.
The specificity of the antibody was confirmed by peptide competition which blocks reaction of the antibody with POU5F1 (lanes 3 and 4, respectively).Immunofluorescence
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — POU5F1
Entrez GeneID
5460Protein Accession#
NP_976034;Q01860Gene Name
POU5F1
Gene Alias
MGC22487, OCT3, OCT4, OTF3, OTF4
Gene Description
POU class 5 homeobox 1
Omim ID
164177Gene Ontology
HyperlinkGene Summary
class 5
Other Designations
OTTHUMP00000029292|POU domain, class 5, transcription factor 1|POU-type homeodomain-containing DNA-binding protein|octamer-binding transcription factor-3
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Interactome
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Disease
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Publication Reference
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Oct-4 mRNA and protein expression during human preimplantation development.
Cauffman G, Van de Velde H, Liebaers I, Van Steirteghem A.
Molecular Human Reproduction 2005 Mar; 11(3):173.
Application：ICC, Human, Oocytes, Preimplantation embryos, Blastocysts.
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Oct-4-expressing cells in human amniotic fluid: a new source for stem cell research?
Prusa AR, Marton E, Rosner M, Bernaschek G, Hengstschlager M.
Human Reproduction 2003 Jul; 18(7):1489.
Application：IF, WB, Human, Amniotic fluid cells.
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High Oct-ane fuel powers the stem cell.
Donovan PJ.
Nature Genetics 2001 Nov; 29(3):246.
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Oct-4 mRNA and protein expression during human preimplantation development.
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