Products

Western Blot (Cell lysate)

Immunoblot of IKBKB polyclonal antibody (Cat # PAB10247) reacted with HeLa cell extract.
All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature.
The membrane was blocked in 5% dry milk for 2 h.
After washing, a 1:500 dilution of the primary antibody was added to the membraneand incubated for 2 h.
Washes with buffer were performed 4 times for 5' each.
The immunoblot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1h.
Washes with TBS preceded color development.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunohistochemical staining with IKBKB polyclonal antibody (Cat # PAB10247) was diluted 1 : 500 to detect IKBKB in human placenta tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

Enzyme-linked Immunoabsorbent Assay

Entrez GeneID

3551

Gene Name

IKBKB

Gene Alias

FLJ40509, IKK-beta, IKK2, IKKB, MGC131801, NFKBIKB

Gene Description

inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta

Omim ID

603258

Gene Ontology

Hyperlink

Gene Summary

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM

Other Designations

inhibitor of nuclear factor kappa B kinase beta subunit|nuclear factor NF-kappa-B inhibitor kinase beta