Western blot using HDAC1 polyclonal antibody (Cat # PAB10217) shows detection of a band at ~65 KDa corresponding to human HDAC1 present in a 293 whole cell lysate (arrowhead). Approximately 35 ug of lysate wseparated on a 4-20% Tris-HEPES gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,350. Reaction occurred 2 h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDyeas™800 conjugated Rb-a-Goat IgG [H&L] MXHu for 45 min at room temperature.
Immunohistochemistry of HDAC1 polyclonal antibody (Cat # PAB10217) shows strong nuclear staining of tumor cells in human lung tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei. Personal Communication, Kenneth Wester, www.proteinatlas.org, Upsala, Sweden.
Immunohistochemistry of HDAC1 polyclonal antibody (Cat # PAB10217) was used at a 1 : 500 dilution to detect HDAC1 by immunohistochemistry in human prostate cancer tissue. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Alan Yen, Life Span Biosciences, Seattle, WA.
Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq