HDAC1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot using HDAC1 polyclonal antibody (Cat # PAB10217) shows detection of a band at ~65 KDa corresponding to human HDAC1 present in a 293 whole cell lysate (arrowhead).
Approximately 35 ug of lysate wseparated on a 4-20% Tris-HEPES gel by SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1:1,350.
Reaction occurred 2 h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDyeas™800 conjugated Rb-a-Goat IgG [H&L] MXHu for 45 min at room temperature.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of HDAC1 polyclonal antibody (Cat # PAB10217) was used at a 1 : 500 dilution to detect HDAC1 by immunohistochemistry in human prostate cancer tissue.
Tissue was formalin-fixed and paraffin embedded.
Personal Communication, Alan Yen, Life Span Biosciences, Seattle, WA.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of HDAC1 polyclonal antibody (Cat # PAB10217) shows strong nuclear staining of tumor cells in human lung tissue.
Tissue was formalin-fixed and paraffin embedded.
Brown color indicates presence of protein, blue color shows cell nuclei.
Personal Communication, Kenneth Wester, www.proteinatlas.org, Upsala, Sweden.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of HDAC1.
Immunogen
A synthetic peptide corresponding to amino acids 466-482 of human HDAC1.
Host
Rabbit
Reactivity
Bovine, Chicken, Chimpanzee, Human, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:10000-1:50000)
Western Blot (1:1000-1:5000)
Immunohistochemistry (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using HDAC1 polyclonal antibody (Cat # PAB10217) shows detection of a band at ~65 KDa corresponding to human HDAC1 present in a 293 whole cell lysate (arrowhead).
Approximately 35 ug of lysate wseparated on a 4-20% Tris-HEPES gel by SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1:1,350.
Reaction occurred 2 h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDyeas™800 conjugated Rb-a-Goat IgG [H&L] MXHu for 45 min at room temperature.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of HDAC1 polyclonal antibody (Cat # PAB10217) was used at a 1 : 500 dilution to detect HDAC1 by immunohistochemistry in human prostate cancer tissue.
Tissue was formalin-fixed and paraffin embedded.
Personal Communication, Alan Yen, Life Span Biosciences, Seattle, WA.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of HDAC1 polyclonal antibody (Cat # PAB10217) shows strong nuclear staining of tumor cells in human lung tissue.
Tissue was formalin-fixed and paraffin embedded.
Brown color indicates presence of protein, blue color shows cell nuclei.
Personal Communication, Kenneth Wester, www.proteinatlas.org, Upsala, Sweden.Enzyme-linked Immunoabsorbent Assay
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Gene Info — HDAC1
Entrez GeneID
3065Protein Accession#
Q13547;NP_004955Gene Name
HDAC1
Gene Alias
DKFZp686H12203, GON-10, HD1, RPD3, RPD3L1
Gene Description
histone deacetylase 1
Omim ID
601241Gene Ontology
HyperlinkGene Summary
Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq
Other Designations
OTTHUMP00000008745|reduced potassium dependency, yeast homolog-like 1
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Interactome
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Pathway
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Disease
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Publication Reference
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ICBP90, an E2F-1 target, recruits HDAC1 and binds to methyl-CpG through its SRA domain.
Unoki M, Nishidate T, Nakamura Y.
Oncogene 2004 Oct; 23(46):7601.
Application：WB, Human, HEK 293T cells.
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Site-specific acetylation of the fetal globin activator NF-E4 prevents its ubiquitination and regulates its interaction with the histone deacetylase, HDAC1.
Zhao Q, Cumming H, Cerruti L, Cunningham JM, Jane SM.
The Journal of Biological Chemistry 2004 Oct; 279(40):41477.
Application：WB, Human, K-562 cells.
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Restoration of transforming growth factor-beta signaling through receptor RI induction by histone deacetylase activity inhibition in breast cancer cells.
Ammanamanchi S, Brattain MG.
The Journal of Biological Chemistry 2004 Jul; 279(31):32620.
Application：WB, Human, MCF-7, ZR75 cells.
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ICBP90, an E2F-1 target, recruits HDAC1 and binds to methyl-CpG through its SRA domain.
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