Product Browser

Last updated: 2014/9/22
  • Related Product Showcase

Product Compare

Product Compare Cancel Click this icon to add products to compare list. Select up to 10 products.

Quick Order (Tutorial)

Input Catalog #,
place order here!
Catalog # :
  • Where to buy
  • Choose your location

HDAC1 polyclonal antibody

  • Catalog # : PAB10217
  • Visit Frequency :
  • Countries :
  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of HDAC1.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 466-482 of human HDAC1.
  • Host:
  • Rabbit
  • Reactivity:
  • Bovine, Chicken, Chimpanzee, Human, Mouse, Rat
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:10000-1:50000)
    Western Blot (1:1000-1:5000)
    Immunohistochemistry (1:200-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using HDAC1 polyclonal antibody (Cat # PAB10217) shows detection of a band at ~65 KDa corresponding to human HDAC1 present in a 293 whole cell lysate (arrowhead).
    Approximately 35 ug of lysate wseparated on a 4-20% Tris-HEPES gel by SDS-PAGE and transferred onto nitrocellulose.
    After blocking the membrane was probed with the primary antibody diluted to 1:1,350.
    Reaction occurred 2 h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDyeas™800 conjugated Rb-a-Goat IgG [H&L] MXHu for 45 min at room temperature.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry of HDAC1 polyclonal antibody (Cat # PAB10217) shows strong nuclear staining of tumor cells in human lung tissue.
    Tissue was formalin-fixed and paraffin embedded.
    Brown color indicates presence of protein, blue color shows cell nuclei.
    Personal Communication, Kenneth Wester, www.proteinatlas.org, Upsala, Sweden.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry of HDAC1 polyclonal antibody (Cat # PAB10217) was used at a 1 : 500 dilution to detect HDAC1 by immunohistochemistry in human prostate cancer tissue.
    Tissue was formalin-fixed and paraffin embedded.
    Personal Communication, Alan Yen, Life Span Biosciences, Seattle, WA.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 3065
  • Protein Accession#:
  • Q13547;NP_004955
  • Gene Name:
  • HDAC1
  • Gene Alias:
  • DKFZp686H12203,GON-10,HD1,RPD3,RPD3L1
  • Gene Description:
  • histone deacetylase 1
  • Gene Summary:
  • Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000008745,reduced potassium dependency, yeast homolog-like 1
  • RSS
  • YouTube
  • Linkedin
  • Facebook