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Western Blot (Cell lysate)

Western blot using BACH1 polyclonal antibody (Cat # PAB10027) shows detection of a band at~105 KDa (Lane 1) corresponding to human BACH1 present in a 293 whole cell lysate (arrowhead).
Lane 2 shows that specific band staining is competed out when the antibody is pre-incubated with the peptide immunogen prior to reaction.
Approximately 35 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1:1,000.
Reaction occurred 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at roomtemperature (800 nm channel, green).
Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).
IRDye™800 fluorescence image was capturedusing the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR,Inc.

Enzyme-linked Immunoabsorbent Assay

Entrez GeneID

571

Protein Accession#

Q9BX63;NP_114432

Gene Name

BACH1

Gene Alias

-

Gene Description

BTB and CNC homology 1, basic leucine zipper transcription factor 1

Omim ID

602751

Gene Ontology

Hyperlink

Gene Summary

This gene encodes a transcription factor that belongs to the cap'n'collar type of basic region leucine zipper factor family (CNC-bZip). The encoded protein contains broad complex, tramtrack, bric-a-brac/poxvirus and zinc finger (BTB/POZ) domains, which is atypical of CNC-bZip family members. These BTB/POZ domains facilitate protein-protein interactions and formation of homo- and/or hetero-oligomers. When this encoded protein forms a heterodimer with MafK, it functions as a repressor of Maf recognition element (MARE) and transcription is repressed. Multiple alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq

Other Designations

BTB and CNC homology 1|OTTHUMP00000096564