Western blot using EGFR polyclonal antibody (Cat # PAB10010) shows detection of aband at ~170 KDa (Lane 1) corresponding to human EGFR present in unstimulated (Lane 1) and EGF (50 ng/mL for 15 min) stimulated (Lane 2) A-431whole cell lysates (arrowhead). Approximately 30 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocell-ulose. After blocking the membrane was probedwith the primary antibody diluted to 1:1,000. Reaction occurred overnight at 4°C followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800nm channel, green). Molecular weight estimationwas made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye™800 fluorescence image was captured usingthe Odyssey® Infrared Imaging System developedby LI-COR. IRDye is a trademark of LI-COR, Inc.
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog,cell growth inhibiting protein 40,cell proliferation-inducing protein 61,epidermal growth factor receptor