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EGFR polyclonal antibody   

  • Catalog # : PAB10010
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  • 100 ug
  • USD $ 415
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of EGFR.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 1189-1199 of human EGFR.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:8000-1:32000)
    Western Blot (1:500-1:5000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using EGFR polyclonal antibody (Cat # PAB10010) shows detection of aband at ~170 KDa (Lane 1) corresponding to human EGFR present in unstimulated (Lane 1) and EGF (50 ng/mL for 15 min) stimulated (Lane 2) A-431whole cell lysates (arrowhead).
    Approximately 30 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocell-ulose.
    After blocking the membrane was probedwith the primary antibody diluted to 1:1,000.
    Reaction occurred overnight at 4°C followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800nm channel, green).
    Molecular weight estimationwas made by comparison to prestained MW markers in lane M (700 nm channel, red).
    IRDye™800 fluorescence image was captured usingthe Odyssey® Infrared Imaging System developedby LI-COR.
    IRDye is a trademark of LI-COR, Inc.
  • Immunohistochemistry
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 1956
  • Protein Accession#:
  • P00533;NP_005219
  • Gene Name:
  • EGFR
  • Gene Alias:
  • ERBB,ERBB1,HER1,PIG61,mENA
  • Gene Description:
  • epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
  • Gene Summary:
  • The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq
  • Other Designations:
  • avian erythroblastic leukemia viral (v-erb-b) oncogene homolog,cell growth inhibiting protein 40,cell proliferation-inducing protein 61,epidermal growth factor receptor
  • Interactome
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