DYKDDDDK tag polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptides of DYKDDDDK tag.
Immunogen
A synthetic peptide corresponding to DYKDDDDK tag.
Host
Rabbit
Specificity
This antibody reacts with proteins containing the Flag epitope tag DYKDDDDK.
Form
Liquid
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.2% gelatin, 0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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The dynamic switch mechanism that leads to activation of LRRK2 is embedded in the DFGψ motif in the kinase domain.
Schmidt SH, Knape MJ, Boassa D, Mumdey N, Kornev AP, Ellisman MH, Taylor SS, Herberg FW.
PNAS 2019 Jul; 116(30):14979.
Application:IF, Human, HEK 293T cells.
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The DDB1-DCAF1-Vpr-UNG2 crystal structure reveals how HIV-1 Vpr steers human UNG2 toward destruction.
Wu Y, Zhou X, Barnes CO, DeLucia M, Cohen AE, Gronenborn AM, Ahn J, Calero G.
Nature Structural & Molecular Biology 2016 Aug; 23(10):933.
Application:IP-WB, Human, HEK293T cells.
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Chronic Contractile Dysfunction without Hypertrophy Does Not Provoke a Compensatory Transcriptional Response in Mouse Hearts.
Matkovich SJ, Grubb DR, McMullen JR, Woodcock EA.
PLoS One 2016 Jun; 11(6):e0158317.
Application:WB-Ti, Mouse, Heart.
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Expressing an inhibitor of PLCβ1b sustains contractile function following pressure overload.
Grubb DR, Gao XM, Kiriazis H, Matsumoto A, McMullen JR, Du XJ, Woodcock EA.
Amino Acids 2016 Feb; 93:12.
Application:WB-Ti, Mouse, Mouse heart.
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Conserved Acidic Amino Acid Residues in a Second RNA Recognition Motif Regulate Assembly and Function of TDP-43.
Shodai A, Ido A, Fujiwara N, Ayaki T, Morimura T, Oono M, Uchida T, Takahashi R, Ito H, Urushitani M.
PLoS One 2012 Dec; 7(12):e52776.
Application:IP-WB, WB, Human, HEK293A.
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LRRK2 Parkinson disease mutations enhance its microtubule association.
Kett LR, Boassa D, Ho CC, Rideout HJ, Hu J, Terada M, Ellisman M, Dauer WT.
Human Molecular Genetics 2012 Feb; 21(4):890.
Application:IF, Human, HEK293T cells.
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Regulation of Na,K-ATPase subunit abundance by translational repression.
Clifford RJ, Kaplan JH.
The Journal of Biological Chemistry 2009 Aug; 284(34):22905.
Application:WB-Tr, Dog, MDCK cells.
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Characterization of a protein phosphatase 2A holoenzyme that dephosphorylates the clathrin adaptors AP-1 and AP-2.
Ricotta D, Hansen J, Preiss C, Teichert D, Honing S.
The Journal of Biological Chemistry 2007 Dec; 283(9):5510.
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The dynamic switch mechanism that leads to activation of LRRK2 is embedded in the DFGψ motif in the kinase domain.
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