ATG3 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot under reducing conditions on 293 cell lysate using rabbit ATG3 polyclonal antibody (Cat # PAB0740) at a dilution of 1 : 50.
Immunofluorescence
Confocal microscopy on immunofluorescently detected ATG3 in cytospin - isolated human white blood cells using rabbit ATG3 polyclonal antibody (Cat # PAB0740) at a dilution of 1 : 100, incubated for 1 hr at room temperature. The cells stained for ATG3 appear in red. The cells were counter stained with Hoechst Dye (blue colour) . Here, the merged picture is presented. More information on the staining : Fixation : In acetone for 90 seconds. Antibody dilutions : Primary antibody was diluted 1 : 100 and incubated for 1 hour, washed then 3x, and the secondary (Goat anti Rb 1 : 100 alexa 546 conjugate) was incubated for 30 minutes. Magnification 80X. No staining was observed using the Pre - immunisation serum. No staining was observed in HL-60 cell line using rabbit ATG3 polyclonal antibody (Cat # PAB0740) .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ATG3.
Immunogen
A synthetic peptide (conjugated with Blue Carrier Protein) corresponding to N-terminus of human ATG3.
Host
Rabbit
Reactivity
Human, Rat
Form
Lyophilized
Recommend Usage
Immunohistochemistry (1:50-1:3000)
Immunofluorescence (1:50-1:3000)
Western Blot (1:50-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Western blot under reducing conditions on 293 cell lysate using rabbit ATG3 polyclonal antibody (Cat # PAB0740) at a dilution of 1 : 50.Immunohistochemistry (Frozen sections)
Immunofluorescence
Confocal microscopy on immunofluorescently detected ATG3 in cytospin - isolated human white blood cells using rabbit ATG3 polyclonal antibody (Cat # PAB0740) at a dilution of 1 : 100, incubated for 1 hr at room temperature. The cells stained for ATG3 appear in red. The cells were counter stained with Hoechst Dye (blue colour) . Here, the merged picture is presented. More information on the staining : Fixation : In acetone for 90 seconds. Antibody dilutions : Primary antibody was diluted 1 : 100 and incubated for 1 hour, washed then 3x, and the secondary (Goat anti Rb 1 : 100 alexa 546 conjugate) was incubated for 30 minutes. Magnification 80X. No staining was observed using the Pre - immunisation serum. No staining was observed in HL-60 cell line using rabbit ATG3 polyclonal antibody (Cat # PAB0740) . -
Gene Info — ATG3
Entrez GeneID
64422Protein Accession#
Q9NT62;Q9CPX6;Q6AZ50;P40344;Gene Name
ATG3
Gene Alias
APG3, APG3-LIKE, APG3L, DKFZp564M1178, FLJ22125, MGC15201, PC3-96
Gene Description
ATG3 autophagy related 3 homolog (S. cerevisiae)
Omim ID
609606Gene Ontology
HyperlinkGene Summary
Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3, a protein-conjugating enzyme essential for autophagy (Tanida et al., 2002 [PubMed 11825910]).[supplied by OMIM
Other Designations
2610016C12Rik|APG3 autophagy 3-like|Apg3p|autophagy Apg3p/Aut1p-like
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