MAP1LC3A polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot on Peripheral Blood Mononuclear Cells (PBMCs) lysate using rabbit MAP1LC3A polyclonal antibody (Cat # PAB0733) at a dilution of 1 : 100 (ECL) .
Immunocytochemistry
Confocal microscopy on immunofluorescently detected MAP1LC3A in cytospin - isolated human white blood cells using rabbit MAP1LC3A polyclonal antibody (Cat # PAB0733) at a dilution of 1 : 200, incubated for 1 h at room temperature. The cells were double - stained for MHC class II appearing in green (A) and MAP1LC3A appearing in red (B) .
Immunofluorescence
Confocal microscopy on immunofluorescently detected MAP1LC3A in cytospin - isolated human white blood cells using rabbit MAP1LC3A polyclonal antibody (Cat # PAB0733) at a dilution of 1 : 200, incubated for 1 hr at room temperature. The cells were double - stained for MAP1LC3A appearing in red and MHC class II appearing in green. The cells were counter stained with Hoechst Dye (blue colour) .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MAP1LC3A.
Immunogen
A synthetic peptide (conjugated with Blue Carrier Protein) corresponding to N-terminus of human MAP1LC3A.
Host
Rabbit
Reactivity
Human, Rat
Form
Lyophilized
Recommend Usage
Immunohistochemistry (1:100-1:1000)
Immunofluorescence (1:100-1:1000)
Western Blot (1:100-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Western blot on Peripheral Blood Mononuclear Cells (PBMCs) lysate using rabbit MAP1LC3A polyclonal antibody (Cat # PAB0733) at a dilution of 1 : 100 (ECL) .Immunohistochemistry (Frozen sections)
Immunocytochemistry
Confocal microscopy on immunofluorescently detected MAP1LC3A in cytospin - isolated human white blood cells using rabbit MAP1LC3A polyclonal antibody (Cat # PAB0733) at a dilution of 1 : 200, incubated for 1 h at room temperature. The cells were double - stained for MHC class II appearing in green (A) and MAP1LC3A appearing in red (B) .Immunofluorescence
Confocal microscopy on immunofluorescently detected MAP1LC3A in cytospin - isolated human white blood cells using rabbit MAP1LC3A polyclonal antibody (Cat # PAB0733) at a dilution of 1 : 200, incubated for 1 hr at room temperature. The cells were double - stained for MAP1LC3A appearing in red and MHC class II appearing in green. The cells were counter stained with Hoechst Dye (blue colour) . -
Gene Info — MAP1LC3A
Entrez GeneID
84557Protein Accession#
Q9H492;Q91VR7;Q6XVN8Gene Name
MAP1LC3A
Gene Alias
LC3, LC3A, MAP1ALC3, MAP1BLC3
Gene Description
microtubule-associated protein 1 light chain 3 alpha
Omim ID
601242Gene Ontology
HyperlinkGene Summary
MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
MAP1 light chain 3-like protein 1|MAP1A/1B light chain 3 A|MAP1A/MAP1B LC3 A|OTTHUMP00000030696|OTTHUMP00000030697|OTTHUMP00000030698|microtubule-associated proteins 1A/1B light chain 3
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