SRC (phospho Y215) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
FG Pancreatic Carcinoma Cell stably expressing vector along (FG-V) the b3 integrin subunit (FG-b3) or a b3 truncation mutant (FG-759x).
SRC (phospho Y215) polyclonal antibody (Cat # PAB0612) was diluted in 1% BSA/TBST and incubated Overnight at 4 °C.
After washing 3x 5 min. with TBST the blots were incubated with Goat anti-mouse or Goat anti-rabbit secondary antibody for 1 hr at room temperature.
The blots were again washed 3x 5 min. with TBST and developed using ECL reagent.
Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of SRC.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y215 of human SRC.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
FG Pancreatic Carcinoma Cell stably expressing vector along (FG-V) the b3 integrin subunit (FG-b3) or a b3 truncation mutant (FG-759x).
SRC (phospho Y215) polyclonal antibody (Cat # PAB0612) was diluted in 1% BSA/TBST and incubated Overnight at 4 °C.
After washing 3x 5 min. with TBST the blots were incubated with Goat anti-mouse or Goat anti-rabbit secondary antibody for 1 hr at room temperature.
The blots were again washed 3x 5 min. with TBST and developed using ECL reagent.
Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD -
Gene Info — SRC
Entrez GeneID
6714Protein Accession#
NP_005408;P12931Gene Name
SRC
Gene Alias
ASV, SRC1, c-SRC, p60-Src
Gene Description
v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
Omim ID
190090Gene Ontology
HyperlinkGene Summary
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000174476|OTTHUMP00000174477|proto-oncogene tyrosine-protein kinase SRC|protooncogene SRC, Rous sarcoma|tyrosine kinase pp60c-src|tyrosine-protein kinase SRC-1
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Interactome
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Pathway
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Disease
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Publication Reference
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Autocrine CSF-1R activation promotes Src-dependent disruption of mammary epithelial architecture.
Wrobel CN, Debnath J, Lin E, Beausoleil S, Roussel MF, Brugge JS.
Journal of Cellular Biology 2004 Apr; 165(2):263.
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Tyrosine 981, a novel ret autophosphorylation site, binds c-Src to mediate neuronal survival.
Encinas M, Crowder RJ, Milbrandt J, Johnson EM Jr.
The Journal of Biological Chemistry 2004 Apr; 279(18):18262.
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Activation of beta-catenin-TCF-mediated transcription by non-receptor tyrosine kinase v-Src.
Haraguchi K, Nishida A, Ishidate T, Akiyama T.
Biochemical and Biophysical Research Communications 2004 Jan; 313(4):841.
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Autocrine CSF-1R activation promotes Src-dependent disruption of mammary epithelial architecture.
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