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Last updated: 2014/8/18
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RAF1 (phospho T269) polyclonal antibody

  • Catalog # : PAB0594
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of RAF1.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T269 of human RAF1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Recommend Usage:
  • ELISA (1:1000)
    Dot Blot (1:100-500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • ELISA
  • Dot Blot (Peptide)
  • Dot Blot (Peptide)
  • Dot blot analysis of RAF1 (phospho T269) polyclonal antibody (Cat # PAB0594) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5 ug/mL. P-Pab : phospho-antibody; P-Peptide : phospho-peptide; NP-Peptide : non-phospho-peptide.
  • Application Image
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 5894
  • Protein Accession#:
  • NP_002871;P04049
  • Gene Name:
  • RAF1
  • Gene Alias:
  • CRAF,NS5,Raf-1,c-Raf
  • Gene Description:
  • v-raf-1 murine leukemia viral oncogene homolog 1
  • Gene Summary:
  • This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq
  • Other Designations:
  • Oncogene RAF1,raf proto-oncogene serine/threonine protein kinase
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