CDKN1A (phospho T145) polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of CDKN1A.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T145 of human CDKN1A.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
Dot Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
CDKN1A (phospho T145) polyclonal antibody (Cat # PAB0555). Western blot analysis of CDKN1A expression in IMR-32.Western Blot (Cell lysate)
The CDKN1A (phospho T145) polyclonal antibody (Cat # PAB0555) is used in Western blot to detect Phospho-CDKN1A-T145 in HeLa cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with CDKN1A (phospho T145) polyclonal antibody (Cat # PAB0555) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.Dot Blot (Peptide)
Dot blot analysis of CDKN1A (phospho T145) polyclonal antibody (Cat # PAB0555) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. -
Gene Info — CDKN1A
Entrez GeneID
1026Protein Accession#
NP_000380;P38936Gene Name
CDKN1A
Gene Alias
CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1
Gene Description
cyclin-dependent kinase inhibitor 1A (p21, Cip1)
Omim ID
116899Gene Ontology
HyperlinkGene Summary
This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported. [provided by RefSeq
Other Designations
CDK-interaction protein 1|DNA synthesis inhibitor|OTTHUMP00000016298|cyclin-dependent kinase inhibitor 1A|melanoma differentiation associated protein 6|wild-type p53-activated fragment 1
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
Methylation status of cyclin-dependent kinase inhibitor genes within the transforming growth factor beta pathway in human T-cell lymphoblastic lymphoma/leukemia.
Scott SA, Kimura T, Dong WF, Ichinohasama R, Bergen S, Kerviche A, Sheridan D, DeCoteau JF.
Leukemia Research 2004 Dec; 28(12):1293.
-
Clusterin-mediated apoptosis is regulated by adenomatous polyposis coli and is p21 dependent but p53 independent.
Chen T, Turner J, McCarthy S, Scaltriti M, Bettuzzi S, Yeatman TJ.
Cancer Research 2004 Oct; 64(20):7412.
-
Interplay between HIV-1 Vpr and Sp1 modulates p21(WAF1) gene expression in human astrocytes.
Amini S, Saunders M, Kelley K, Khalili K, Sawaya BE.
The Journal of Biological Chemistry 2004 Aug; 279(44):46046.
-
Methylation status of cyclin-dependent kinase inhibitor genes within the transforming growth factor beta pathway in human T-cell lymphoblastic lymphoma/leukemia.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com