KDR (phospho Y996) polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
KDR (phospho Y996) polyclonal antibody (Cat # PAB0505) is used in Western blot to detect Phospho-KDR-Y996 in HMVEC cell lysate. Endothelial cells were stimulated with 50 ug/mL VEGF for 5min; 20 ug lysate from HMVEC was loaded onto an 8% gel; for Western blot, membranes were incubated O/N with KDR (phospho Y996) polyclonal antibody (Cat # PAB0505) diluted in 1% Milk/TBST. Data and Protocol kindly provided by Dr. Weis from Cheresh Lab, UCSD.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with KDR (phospho Y996) polyclonal antibody (Cat # PAB0505) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of KDR.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y996 of human KDR.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
KDR (phospho Y996) polyclonal antibody (Cat # PAB0505) is used in Western blot to detect Phospho-KDR-Y996 in HMVEC cell lysate. Endothelial cells were stimulated with 50 ug/mL VEGF for 5min; 20 ug lysate from HMVEC was loaded onto an 8% gel; for Western blot, membranes were incubated O/N with KDR (phospho Y996) polyclonal antibody (Cat # PAB0505) diluted in 1% Milk/TBST. Data and Protocol kindly provided by Dr. Weis from Cheresh Lab, UCSD.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with KDR (phospho Y996) polyclonal antibody (Cat # PAB0505) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — KDR
Entrez GeneID
3791Protein Accession#
NP_002244;P35968Gene Name
KDR
Gene Alias
CD309, FLK1, VEGFR, VEGFR2
Gene Description
kinase insert domain receptor (a type III receptor tyrosine kinase)
Gene Ontology
HyperlinkGene Summary
Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq
Other Designations
soluble VEGFR2|vascular endothelial growth factor receptor 2
-
Interactome
-
Pathway
-
Disease
- Adenocarcinoma
- Alzheimer disease
- Angina Pectoris
- Anoxia
- Asthma
- Brain Neoplasms
- Breast cancer
- Breast Neoplasms
- Carcinoma
+ View More Disease
-
Publication Reference
-
Platelet factor 4 disrupts the intracellular signalling cascade induced by vascular endothelial growth factor by both KDR dependent and independent mechanisms.
Sulpice E, Contreres JO, Lacour J, Bryckaert M, Tobelem G.
European Journal of Biochemistry 2004 Aug; 271(16):3310.
-
Regulation of vascular endothelial growth factor receptor 2-mediated phosphorylation of focal adhesion kinase by heat shock protein 90 and Src kinase activities.
Le Boeuf F, Houle F, Huot J.
The Journal of Biological Chemistry 2004 Sep; 279(37):39175.
-
VEGF receptor phosphorylation status and apoptosis is modulated by a green tea component, epigallocatechin-3-gallate (EGCG), in B-cell chronic lymphocytic leukemia.
Lee YK, Bone ND, Strege AK, Shanafelt TD, Jelinek DF, Kay NE.
Blood 2004 Aug; 104(3):788.
-
Platelet factor 4 disrupts the intracellular signalling cascade induced by vascular endothelial growth factor by both KDR dependent and independent mechanisms.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com