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Last updated: 2014/10/27
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HIST1H3B (phospho S10) polyclonal antibody

  • Catalog # : PAB0488
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of HIST1H3B.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with MAPS) corresponding to residues surrounding S10 of human HIST1H3B.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • ELISA (1:1000)
    Dot Blot (1:500)
    Immunohistochemistry (1:50-100)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with HIST1H3B (phospho S10) polyclonal antibody (Cat # PAB0488) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
  • ELISA
  • Dot Blot (Peptide)
  • Dot Blot (Peptide)
  • Dot blot analysis of HIST1H3B (phospho S10) polyclonal antibody (Cat # PAB0488) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL.
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
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  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 8358
  • Protein Accession#:
  • NP_003528;P68431
  • Gene Name:
  • HIST1H3B
  • Gene Alias:
  • H3/l,H3FL
  • Gene Description:
  • histone cluster 1, H3b
  • Gene Summary:
  • Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq
  • Other Designations:
  • H3 histone family, member L,OTTHUMP00000016132,histone 1, H3b
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