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Last updated: 2014/11/23
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CDC2 (phospho T161) polyclonal antibody

  • Catalog # : PAB0434
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of CDC2.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T161 of human CDC2.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • ELISA (1:1000)
    Western Blot (1:100-500)
    Immunohistochemistry (1:50-100)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western blot analysis of CDC2 (phospho T161) polyclonal antibody (Cat # PAB0434) in mouse brain tissue lysate (35ug/lane). Phospho-CDC2 (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of CDC2 (phospho T161) polyclonal antibody (Cat # PAB0434). HEK-293 cell line lysate (1 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDC2 gene (Lane 2). CDC2 (phospho T161) polyclonal antibody (arrow) was detected using the purified Pab (1:1500 dilution).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human cancer tissue reacted with CDC2 (phospho T161) polyclonal antibody (Cat # PAB0434) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 983
  • Protein Accession#:
  • NP_001777;P06493
  • Gene Name:
  • CDC2
  • Gene Alias:
  • CDC28A,CDK1,DKFZp686L20222,MGC111195
  • Gene Description:
  • cell division cycle 2, G1 to S and G2 to M
  • Gene Summary:
  • The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000019660,cell cycle controller CDC2,cell division control protein 2 homolog,cell division cycle 2 protein,cyclin-dependent kinase 1,p34 protein kinase
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