BAD (phospho S75) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
The BAD (phospho S75) polyclonal antibody (Cat # PAB0417) is used in Western blot to detect Phospho-BAD-S75 in HL-60 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with BAD (phospho S75) polyclonal antibody (Cat # PAB0417) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of BAD.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S75 of human BAD.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The BAD (phospho S75) polyclonal antibody (Cat # PAB0417) is used in Western blot to detect Phospho-BAD-S75 in HL-60 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with BAD (phospho S75) polyclonal antibody (Cat # PAB0417) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — BAD
Entrez GeneID
572Protein Accession#
NP_116784;Q92934Gene Name
BAD
Gene Alias
BBC2, BCL2L8
Gene Description
BCL2-associated agonist of cell death
Omim ID
603167Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq
Other Designations
BCL-X/BCL-2 binding protein|BCL2-antagonist of cell death protein|BCL2-binding component 6|BCL2-binding protein
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Interactome
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Pathway
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Disease
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Publication Reference
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A chimeric protein induces tumor cell apoptosis by delivering the human Bcl-2 family BH3-only protein Bad.
Antignani A, Youle RJ.
Biochemistry 2005 Mar; 44(10):4074.
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Broad degradation of proapoptotic proteins with the conserved Bcl-2 homology domain 3 during infection with Chlamydia trachomatis.
Ying S, Seiffert BM, Häcker G, Fischer SF.
Infection and Immunity 2005 Mar; 73(3):1399.
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Cooperation of amphiregulin and insulin-like growth factor-1 inhibits Bax- and Bad-mediated apoptosis via a protein kinase C-dependent pathway in non-small cell lung cancer cells.
Hurbin A, Coll JL, Dubrez-Daloz L, Mari B, Auberger P, Brambilla C, Favrot MC.
The Journal of Biological Chemistry 2005 May; 280(20):19757.
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A chimeric protein induces tumor cell apoptosis by delivering the human Bcl-2 family BH3-only protein Bad.
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