YES1 (T348I) (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human YES1 (NP_005424.1, 1 a.a. - 543 a.a.) T348I mutant full length recombinant protein with GST tag expressed in Baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
88
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
82 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
Substrate : Srctide. ATP: 100 µM.Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — YES1
Entrez GeneID
7525Protein Accession#
NP_005424.1Gene Name
YES1
Gene Alias
HsT441, P61-YES, Yes, c-yes
Gene Description
v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1
Omim ID
164880Gene Ontology
HyperlinkGene Summary
This gene is the cellular homolog of the Yamaguchi sarcoma virus oncogene. The encoded protein has tyrosine kinase activity and belongs to the src family of proteins. This gene lies in close proximity to thymidylate synthase gene on chromosome 18, and a corresponding pseudogene has been found on chromosome 22. [provided by RefSeq
Other Designations
OTTHUMP00000162194|Yamaguchi sarcoma oncogene|cellular yes-1 protein|proto-oncogene tyrosine-protein kinase YES|viral oncogene yes-1 homolog 1
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