STK25 (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human STK25 (NP_006365.2, 1 a.a. - 426 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
75
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
98 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: Moesin-derived peptide. ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — STK25
Entrez GeneID
10494Protein Accession#
NP_006365.2Gene Name
STK25
Gene Alias
DKFZp686J1430, SOK1, YSK1
Gene Description
serine/threonine kinase 25 (STE20 homolog, yeast)
Omim ID
602255Gene Ontology
HyperlinkGene Summary
O
Other Designations
Ste20, yeast homolog|serine/threonine kinase 25|serine/threonine kinase 25 (Ste20, yeast homolog)|sterile 20 (oxidant stress response kinase 1; yeast Sps1/Ste20-related kinase 1)
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