PDK4 (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human PDK4 (NP_002603.1, 1 a.a. - 411 a.a.) full-length recombinant protein with GST tag expressed in Escherichia coli.
Host
Escherichia coli
Theoretical MW (kDa)
73
Form
Liquid
Preparation Method
Escherichia coli expression system
Purification
Glutathione sepharose chromatography
Purity
77 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: PDHKtide. ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — PDK4
Entrez GeneID
5166Protein Accession#
NP_002603.1Gene Name
PDK4
Gene Alias
FLJ40832
Gene Description
pyruvate dehydrogenase kinase, isozyme 4
Omim ID
602527Gene Ontology
HyperlinkGene Summary
This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin. [provided by RefSeq
Other Designations
pyruvate dehydrogenase kinase 4|pyruvate dehydrogenase kinase, isoenzyme 4
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