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BIOtech
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KIT (T670I) (Human) Recombinant ProteinBioActive

  • Catalog # : P5571
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  • Specification
  • Product Description:
  • Human KIT (NP_000213.1, 544 a.a. - 976 a.a.) T670I mutant partial recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
  • Host:
  • Insect
  • Theoretical MW (kDa):
  • 76
  • Form:
  • Liquid
  • Preparation Method:
  • Baculovirus infected insect cell (Sf21) expression system
  • Purification:
  • Glutathione sepharose chromatography
  • Purity:
  • 62 % by SDS-PAGE/CBB staining
  • Activity:
  • The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: Srctide. ATP: 1000 uM
  • Quality Control Testing:
  • Loading 1 ug protein in SDS-PAGE

    QC Testing of P5571
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 3815
  • Gene Name:
  • KIT
  • Gene Alias:
  • C-Kit,CD117,PBT,SCFR
  • Gene Description:
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
  • Gene Summary:
  • This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • mast/stem cell growth factor receptor,proto-oncogene tyrosine-protein kinase Kit,soluble KIT variant 1
  • Interactome
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