CDC2L6/CCNC (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human CDC2L6 (NP_055891.1, 1 a.a. - 502 a.a.) and CCNC (NP_005181.2, 1 a.a. - 283 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
85
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
92 % by SDS-PAGE/CBB staining
Activity
The activity was determined by ELISA. The enzyme was incubated with biotinylated substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into streptavidin- coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG. Substrate: RNA polymerase peptide. ATP: 150 uM
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — CCNC
Entrez GeneID
892Protein Accession#
NP_055891.1 (Gene ID : 23097);NP_005181.2 (Gene ID : 892)Gene Name
CCNC
Gene Alias
CycC
Gene Description
cyclin C
Omim ID
123838Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the cyclin family of proteins. The encoded protein interacts with cyclin-dependent kinase 8 and induces the phophorylation of the carboxy-terminal domain of the large subunit of RNA polymerase II. The level of mRNAs for this gene peaks in the G1 phase of the cell cycle. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000016897
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Gene Info — CDC2L6
Entrez GeneID
23097Protein Accession#
NP_055891.1 (Gene ID : 23097);NP_005181.2 (Gene ID : 892)Gene Name
CDC2L6
Gene Alias
CDK11, KIAA1028, bA346C16.3
Gene Description
cell division cycle 2-like 6 (CDK8-like)
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that is one of the components of the Mediator coactivator complex. The Mediator complex is a multiprotein complex required for transcriptional activation by DNA binding transcription factors of genes transcribed by RNA polymerase II. The protein encoded by this gene is similar to cyclin-dependent kinase 8 which can also be a component of the Mediator complex. [provided by RefSeq
Other Designations
CDK8-like cyclin-dependent kinase|OTTHUMP00000017005|OTTHUMP00000040460|cyclin-dependent kinase (CDC2-like) 11|death-preventing kinase
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Interactome
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Disease
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