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BIOtech
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CAMK2D (Human) Recombinant ProteinBioActive

  • Catalog # : P5506
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  • Specification
  • Product Description:
  • Human CAMK2D (NP_742113.1, 1 a.a. - 478 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
  • Host:
  • Insect
  • Theoretical MW (kDa):
  • 81
  • Form:
  • Liquid
  • Preparation Method:
  • Baculovirus infected insect cell (Sf21) expression system
  • Purification:
  • Glutathione sepharose chromatography
  • Purity:
  • 82 % by SDS-PAGE/CBB staining
  • Activity:
  • The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate, Mg(or Mn)/ATP, and Ca/Calmodulin . The phosphorylated and unphosphorylated substrates were separated and detected by LabChip TM3000. Substrate: GS peptide. ATP: 100 uM.
  • Quality Control Testing:
  • Loading 1 ug protein in SDS-PAGE

    QC Testing of P5506
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 817
  • Gene Name:
  • CAMK2D
  • Gene Alias:
  • CAMKD,DKFZp686G23119,DKFZp686I2288,MGC44911
  • Gene Description:
  • calcium/calmodulin-dependent protein kinase II delta
  • Gene Summary:
  • The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a delta chain. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Distinct isoforms of this chain have different expression patterns
  • Other Designations:
  • CaM kinase II delta subunit,CaM-kinase II delta chain,CaMK-II delta subunit,OTTHUMP00000163829,OTTHUMP00000163830,calcium/calmodulin-dependent protein kinase (CaM kinase) II delta,calcium/calmodulin-dependent protein kinase type II delta chain
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