AS3MT (Human) Recombinant Protein
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Specification
Product Description
Human AS3MT (NP_065733, 1 a.a. - 375 a.a.) full-length recombinant protein with His tag expressed in Escherichia coli.
Sequence
MGSSHHHHHHSSGLVPRGSHMGSHMAALRDAEIQKDVQTYYGQVLKRSADLQTNGCVTTARPVPKHIREALQNVHEEVALRYYGCGLVIPEHLENCWILDLGSGSGRDCYVLSQLVGEKGHVTGIDMTKGQVEVAEKYLDYHMEKYGFQASNVTFIHGYIEKLGEAGIKNESHDIVVSNCVINLVPDKQQVLQEAYRVLKHGGELYFSDVYTSLELPEEIRTHKVLWGECLGGALYWKELAVLAQKIGFCPPRLVTANLITIQNKELERVIGDCRFVSATFRLFKHSKTGPTKRCQVIYNGGITGHEKELMFDANFTFKEGEIVEVDEETAAILKNSRFAQDFLIRPIGEKLPTSGGCSALELKDIITDPFKLAEESDSMKSRCVPDAAGGCCGTKKSC
Host
Escherichia coli
Theoretical MW (kDa)
44.3
Form
Liquid
Preparation Method
Escherichia coli expression system
Purification
Conventional Chromatography
Concentration
1 mg/mL
Purity
> 90% by SDS-PAGE
Quality Control Testing
Loading 3 ug protein in 15% SDS-PAGE
Storage Buffer
In 20 mM Tris-HCl buffer, 0.15 M NaCl, pH 8.0 (10% glycerol).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
SDS-PAGE
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Gene Info — AS3MT
Entrez GeneID
57412Protein Accession#
NP_065733Gene Name
AS3MT
Gene Alias
CYT19
Gene Description
arsenic (+3 oxidation state) methyltransferase
Gene Ontology
HyperlinkGene Summary
AS3MT catalyzes the transfer of a methyl group from S-adenosyl-L-methionine (AdoMet) to trivalent arsenical and may play a role in arsenic metabolism (Lin et al., 2002 [PubMed 11790780]).[supplied by OMIM
Other Designations
2310045H08Rik|OTTHUMP00000020384|S-adenosylmethionine:arsenic (III) methyltransferase|methylarsonite methyltransferase|methyltransferase cyt19
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Interactome
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Disease
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Publication Reference
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Interactive Effects of N6AMT1 and As3MT in Arsenic Biomethylation.
Zhang H, Ge Y, He P, Chen X, Carina A, Qiu Y, Aga DS, Ren X.
Toxicological Sciences 2015 Aug; 146(2):354.
Application:SDS-PAGE, Human, UROtsa cells.
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Interactive Effects of N6AMT1 and As3MT in Arsenic Biomethylation.
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